易感西葫芦子叶对黄瓜花叶病毒感染的复杂空间反应。
Complex spatial responses to cucumber mosaic virus infection in susceptible Cucurbita pepo cotyledons.
作者信息
Havelda Z, Maule A J
机构信息
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom.
出版信息
Plant Cell. 2000 Oct;12(10):1975-86. doi: 10.1105/tpc.12.10.1975.
Abstract
Cucumber mosaic virus infection of its susceptible host Cucurbita pepo results in a program of biochemical changes after virus infection. Applying a spatial analysis to expanding infected lesions, we investigated the relationship between the changes in enzyme activity and gene expression. Patterns of altered expression were seen that could not be detected by RNA gel blot analysis. For all the host genes studied, there was a downregulation (shutoff) of expression within the lesion. In addition, two distinct types of upregulation were observed. The expression of heat shock protein 70 (HSP70) and NADP(+)-dependent malic enzyme (NADP-ME) showed induction in apparently uninfected cells ahead of the infection. This response was more localized than the upregulation exhibited by catalase expression, which occurred throughout the uninfected regions of the tissue. The experiments showed that virus infection induced immediate and subsequent changes in gene expression by the host and that the infection has the potential to give advance signaling of the imminent infection.
摘要
黄瓜花叶病毒感染其易感宿主西葫芦后，病毒感染会引发一系列生化变化。通过对不断扩大的感染病斑进行空间分析，我们研究了酶活性变化与基因表达之间的关系。我们发现了一些通过RNA凝胶印迹分析无法检测到的表达改变模式。对于所有研究的宿主基因，病斑内的表达均出现下调（关闭）。此外，还观察到两种不同类型的上调。热休克蛋白70（HSP70）和依赖烟酰胺腺嘌呤二核苷酸磷酸（NADP(+)）的苹果酸酶（NADP-ME）的表达在明显未感染的细胞中先于感染出现诱导。这种反应比过氧化氢酶表达所呈现的上调更为局限，过氧化氢酶表达的上调发生在组织的未感染区域。实验表明，病毒感染诱导宿主基因表达立即发生及随后的变化，并且这种感染有可能对即将发生的感染发出提前信号。
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