Thompson Jeremy R, Doun Stephanie, Perry Keith L
Department of Plant Pathology, Cornell University, Ithaca, NY 14850, USA.
J Virol. 2006 Aug;80(15):7740-3. doi: 10.1128/JVI.00436-06.
Cucumber mosaic virus (CMV) systemically infects both tobacco and zucchini squash. CMV capsid protein loop mutants with single-amino-acid substitutions are unable to systemically infect squash, but they revert to a wild-type phenotype in the presence of an additional, specific single-site substitution. The D118A, T120A, D192A, and D197A loop mutants reverted to a wild-type phenotype but did so in combination with P56S, P77L, A162V, and I53F or T124I mutations, respectively. The possible effect of these compensatory mutations on other, nonsystemically infecting loop mutants was tested with the F117A mutant and found to be neutral, thus indicating a specificity to the observed changes.
黄瓜花叶病毒（CMV）可系统性感染烟草和西葫芦。具有单氨基酸替换的CMV衣壳蛋白环突变体无法系统性感染西葫芦，但在存在另一个特定单点替换的情况下会恢复为野生型表型。D118A、T120A、D192A和D197A环突变体分别与P56S、P77L、A162V和I53F或T124I突变组合时恢复为野生型表型。用F117A突变体测试了这些补偿性突变对其他非系统性感染环突变体的可能影响，结果发现是中性的，因此表明对观察到的变化具有特异性。
