虹鳟鱼（鲑鱼）5S核糖体核糖核酸的一种拟议核苷酸序列。
A proposed nucleotide sequence for the 5S ribosomal ribonucleic acid of rainbow trout (Salmo gairdneri).
作者信息
Roy K L
出版信息
Can J Biochem. 1978 Jan;56(1):60-5. doi: 10.1139/o78-009.
DOI:10.1139/o78-009
PMID:110424
Abstract
Rainbow trout cell cultures have been exposed to 32P-labelled inorganic phosphate and the labelled RNA has been isolated. The 5S ribosomal ribonucleic acid (5S rRNA) was purified by polyacrylamide gel electrophoresis, then digested with RNase T1 or pancreatic RNase. The products of complete digestion were separated and their sequences determined. These analyses have allowed a sequence to be proposed which differs in eight positions from that of mammalian 5S rRNAs.
摘要
虹鳟鱼细胞培养物已暴露于32P标记的无机磷酸盐，并分离出了标记的RNA。5S核糖体核糖核酸（5S rRNA）通过聚丙烯酰胺凝胶电泳纯化，然后用核糖核酸酶T1或胰核糖核酸酶消化。完全消化的产物被分离并确定其序列。这些分析得出了一个与哺乳动物5S rRNA在八个位置上不同的序列。
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