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通过5'-末端标记、部分消化和同系层析指纹图谱对18S核糖体RNA的T1核糖核酸酶片段进行序列分析。

Sequence analysis of T1 ribonuclease fragments of 18S ribosomal RNA by 5'-terminal labeling, partial digestion, and homochromatography fingerprinting.

作者信息

Fuke M, Busch H

出版信息

Nucleic Acids Res. 1977 Feb;4(2):339-52. doi: 10.1093/nar/4.2.339.

DOI:10.1093/nar/4.2.339
PMID:190590
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342436/
Abstract

The method employed to determine the sequence of a T1 RNase fragment, A-A-A-A-A-U-A-A-C-A-A-U-A-C-A-Gp, from Novikoff rat hepatoma 18S ribosomal RNA is described. This method is applicable to any oligoribonucleotide produced by specific endonucleases that leave the newly cleaved 5'-end free for labeling with polynucleotide kinase and gamma-(32p)-ATP. The (32p)-labeled oligoribonucleotide is subjected to partial endonucleolytic digestion and fractionated by two-dimensional homochromatography fingerprinting. The nucleotide sequence is determined by following mobility shifts of the labeled and partially digested oligoribonucleotides in homochromatography fingerprinting.

摘要

本文描述了用于确定来自诺维科夫大鼠肝癌18S核糖体RNA的T1核糖核酸酶片段A-A-A-A-A-U-A-A-C-A-A-U-A-C-A-Gp序列的方法。该方法适用于由特异性内切核酸酶产生的任何寡核糖核苷酸，这些酶使新切割的5'-末端游离，以便用多核苷酸激酶和γ-(32P)-ATP进行标记。将(32P)标记的寡核糖核苷酸进行部分内切核酸酶消化，并通过二维同系层析指纹图谱进行分级分离。通过跟踪标记的和部分消化的寡核糖核苷酸在同系层析指纹图谱中的迁移率变化来确定核苷酸序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/b3a48a7f1aff/nar00475-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/aab2414d2122/nar00475-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/110d0075f0de/nar00475-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/588bc72f29d4/nar00475-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/2833a4549078/nar00475-0079-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/ff4ffcf7fe94/nar00475-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/df08526be0ee/nar00475-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/b3a48a7f1aff/nar00475-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/aab2414d2122/nar00475-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/110d0075f0de/nar00475-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/588bc72f29d4/nar00475-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/2833a4549078/nar00475-0079-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/ff4ffcf7fe94/nar00475-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/df08526be0ee/nar00475-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f251/342436/b3a48a7f1aff/nar00475-0084-a.jpg

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Sequence analysis of T1 ribonuclease fragments of 18S ribosomal RNA by 5'-terminal labeling, partial digestion, and homochromatography fingerprinting.通过5'-末端标记、部分消化和同系层析指纹图谱对18S核糖体RNA的T1核糖核酸酶片段进行序列分析。
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本文引用的文献

1
The structure of ribonucleic acid. I. Cyclic nucleotides produced by ribonuclease and by alkaline hydrolysis.核糖核酸的结构。I. 核糖核酸酶及碱性水解产生的环核苷酸。
Biochem J. 1952 Dec;52(4):552-7. doi: 10.1042/bj0520552.
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Studies on extracellular ribonucleases of Ustilago sphaerogena. Characterization of substrate specificity with special reference to purine-specific ribonucleases.黑粉菌胞外核糖核酸酶的研究。底物特异性的表征，特别提及嘌呤特异性核糖核酸酶。
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Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids.
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多核苷酸激酶在非放射性核酸指纹分析中的应用。
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Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids 1. the 5'-termini of Excherichia coli ribosomal RNA.核糖核酸5′末端核苷酸序列分析。1. 大肠杆菌核糖体RNA的5′末端
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Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65. doi: 10.1073/pnas.54.1.158.
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Ribonucleases in taka-diastase: properties, chemical nature, and applications.淀粉酶中的核糖核酸酶：特性、化学性质及应用
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