[黄曲霉毒素B1多克隆抗体和单克隆抗体的比较免疫化学特性]
[Comparative immunochemical characteristics of polyclonal and monoclonal antibodies to aflatoxin B1].
作者信息
Burkin M A, Iakovleva I V, Sviridov V V, Burkin A A, Kononenko G P, Soboleva H A
机构信息
Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia.
出版信息
Prikl Biokhim Mikrobiol. 2000 Sep-Oct;36(5):575-81.
Four hybrid clones (MM-(AB1)-1, MM-(AB1)-2, MM-(AB1)-3, and MM-(AB1)-4) were obtained by hybridoma technology with immunization of BALB/c mice with a BSA conjugate of aflatoxin B1 carboxymethyloxime derivative. Antibodies produced by these clones varied in the ability to recognize the aflatoxin B1 analogues. The sensitivity of enzyme immunoassay based on all monoclonal antibodies was higher compared to analysis based on polyclonal rabbit antibodies (0.1 and 0.4 ng/ml, respectively).
用黄曲霉毒素B1羧甲基肟衍生物的牛血清白蛋白偶联物免疫BALB/c小鼠，通过杂交瘤技术获得了四个杂交克隆（MM-(AB1)-1、MM-(AB1)-2、MM-(AB1)-3和MM-(AB1)-4）。这些克隆产生的抗体在识别黄曲霉毒素B1类似物的能力上有所不同。与基于兔多克隆抗体的分析相比，基于所有单克隆抗体的酶免疫测定的灵敏度更高（分别为0.1和0.4 ng/ml）。
Zotero 插件