Shim Won-Bo, Yang Zheng-You, Kim Jung-Sook, Kim Ji-Young, Kang Sung-Jo, Woo Gun-Jo, Chung Young-Chul, Eremin Sergei A, Chung Duck-Hwa
Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University, Jinju, Korea.
J Microbiol Biotechnol. 2007 Oct;17(10):1629-37.
An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.
开发并优化了一种使用纳米胶体金-抗体探针的免疫层析（ICG）试纸条检测方法，用于快速检测黄曲霉毒素B1（AFB1）。从克隆的杂交瘤细胞（AF78）中制备了对AFB1特异的单克隆抗体，将其与纳米胶体金偶联，并分布在ICG试纸条检测的结合垫上。ICG试纸条检测的目视检测限为0.5 ng/ml，该方法对黄曲霉毒素B2、G1和G2有交叉反应。总共收集了172份谷物和饲料样品，并通过ICG试纸条检测和高效液相色谱法（HPLC）进行分析。ICG试纸条检测的结果与HPLC获得的结果显示出良好的一致性。这些结果表明，ICG试纸条检测作为一种快速且经济高效的筛选工具，可用于实际样品中AFB1的测定，并且可应用于食品和农产品中霉菌毒素的初步筛选，无需复杂步骤即可在15分钟内得出结果。
