Sakai K, Crochet S
INSERM U480, Département de Médecine Expérimentale, Université Claude Bernard, Lyon, France.
Neuroreport. 2000 Sep 28;11(14):3237-41. doi: 10.1097/00001756-200009280-00037.
Using in vivo extracellular unit recordings combined with microdialysis infusion in the cat, we found that the cessation of discharge of presumed serotonergic dorsal raphe neurons during paradoxical sleep (PS) was completely blocked by either histamine or phenylephrine, an alpha1 adrenoceptor agonist, but not by bicuculline, a GABA receptor antagonist. In addition, application of mepyramine, a specific H1 histamine receptor antagonist, or prazosin, a specific alpha1 adrenoceptor antagonist, suppressed the spontaneous discharge of raphe neurons during both quiet waking and sleep. The present data suggest that this cessation of dorsal raphe unit activity is caused by the mechanism of disfacilitation resulting from the cessation of discharge of norepinephrine- or histamine-containing neurons during PS.
通过在猫身上结合微透析灌注进行体内细胞外单位记录，我们发现，在异相睡眠（PS）期间，假定的5-羟色胺能中缝背核神经元放电停止完全被组胺或苯肾上腺素（一种α1肾上腺素能受体激动剂）阻断，但未被GABA受体拮抗剂荷包牡丹碱阻断。此外，应用特异性H1组胺受体拮抗剂美吡拉敏或特异性α1肾上腺素能受体拮抗剂哌唑嗪，可抑制安静觉醒和睡眠期间中缝核神经元的自发放电。目前的数据表明，中缝背核单位活动的这种停止是由PS期间去甲肾上腺素能或组胺能神经元放电停止导致的去易化机制引起的。
