Moore A H, Osteen C L, Chatziioannou A F, Hovda D A, Cherry S R
Division of Neurosurgery, University of California, Los Angeles, USA.
J Cereb Blood Flow Metab. 2000 Oct;20(10):1492-501. doi: 10.1097/00004647-200010000-00011.
With the advent and emerging importance of neurobiology and its relation to behavior, scientists desire the capability to apply noninvasive, quantitative imaging of neuronal activity to small rodents. To this end, the authors' laboratory has developed microPET, a high-resolution positron emission tomography (PET) scanner that is capable of performing in vivo molecular imaging at a resolution sufficient to resolve major structures in the rat brain. The authors report in this article that, in conjunction with 2-[18F]fluoro-2-deoxyglucose (FDG), microPET provides accurate rates of cerebral glucose metabolism (59.7 to 108.5 micromol/100 g x min) in conscious adult rats as validated by within-subject autoradiographic measurements (59.5 to 136.2 micromol/100 g x min; r = 0.88; F[1,46] = 168.0; P < 0.001). By conducting repeated quantitative scanning, the authors demonstrate the sensitivity and accuracy of FDG-microPET to detect within-subject metabolic changes induced by traumatic brain injury. In addition, the authors report that longitudinal recovery from traumatic brain injury-induced metabolic depression, as measured by quantitative FDG-microPET, is significantly correlated (r = 0.65; P < 0.05) to recovery of behavioral dysfunction, as assessed by the Morris Water Maze performance of the same rats, after injury. This is the first study to demonstrate that FDG-microPET is quantitative, reproducible, and sensitive to metabolic changes, introducing a new approach to the longitudinal study of small animal models in neuroscience research.
随着神经生物学的出现及其与行为关系的日益重要，科学家们希望能够对小型啮齿动物进行无创、定量的神经元活动成像。为此，作者所在的实验室开发了微型正电子发射断层扫描仪（microPET），这是一种高分辨率正电子发射断层扫描（PET）扫描仪，能够以足以分辨大鼠大脑主要结构的分辨率进行体内分子成像。作者在本文中报告称，与2-[18F]氟-2-脱氧葡萄糖（FDG）结合使用时，microPET可在清醒成年大鼠中提供准确的脑葡萄糖代谢率（59.7至108.5微摩尔/100克×分钟），经受试者自身放射自显影测量验证（59.5至136.2微摩尔/100克×分钟；r = 0.88；F[1,46] = 168.0；P < 0.001）。通过进行重复定量扫描，作者证明了FDG-microPET检测创伤性脑损伤引起的受试者体内代谢变化的敏感性和准确性。此外，作者报告称，通过定量FDG-microPET测量，创伤性脑损伤引起的代谢抑制的纵向恢复与同一大鼠受伤后通过莫里斯水迷宫表现评估的行为功能障碍的恢复显著相关（r = 0.65；P < 0.05）。这是第一项证明FDG-microPET具有定量性、可重复性且对代谢变化敏感的研究，为神经科学研究中的小动物模型纵向研究引入了一种新方法。
