Koyama H, Iwata H, Kuwabara Y, Iwase H, Kobayashi S, Fujii Y
Department of Surgery II, Nagoya City University Medical School, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, 467-8801, Aichi, Japan.
Eur J Cancer. 2000 Oct;36(16):2164-70. doi: 10.1016/s0959-8049(00)00297-5.
In this study, we investigated the activity of matrix metalloproteinase (MMP)-2 and -9 by gelatine zymography, immunostaining and in situ gelatine zymography in 30 oesophageal squamous-cell carcinomas. The gelatinolytic activity in situ was detected in all cases with different patterns of localisation. Significant gelatinolysis by stromal cells adjacent to tumour nests was found in 12 cases. Strong gelatinolytic activity appeared within the tumour nest itself in 13 cases. In the other 5 cases, both stromal cells and tumour cells showed the gelatinolytic activity. Gelatine zymography demonstrated a correlation between vascular invasion and activation of MMP-9. It also demonstrated a correlation between lymph node metastasis, lymphatic or vascular invasion and activation of MMP-2. These results suggest that MMPs play an important role in the invasion of oesophageal carcinoma.
在本研究中，我们通过明胶酶谱法、免疫染色和原位明胶酶谱法，对30例食管鳞状细胞癌中基质金属蛋白酶（MMP）-2和-9的活性进行了研究。在所有病例中均检测到原位明胶溶解活性，其定位模式各异。在12例病例中，发现肿瘤巢邻近的基质细胞有显著的明胶溶解现象。13例病例中，肿瘤巢内部出现强烈的明胶溶解活性。在另外5例病例中，基质细胞和肿瘤细胞均表现出明胶溶解活性。明胶酶谱法显示MMP-9的激活与血管侵犯之间存在相关性。它还显示MMP-2的激活与淋巴结转移、淋巴管或血管侵犯之间存在相关性。这些结果表明，基质金属蛋白酶在食管癌的侵袭过程中起重要作用。
