Culture of cells in beem capsules: a new technique for electron microscopic study of monolayer cultures.

A method is described for the monolayer cultivation of primary cell suspensions and established cell lines directly in carbon-coated BEEM capsules, BEEM capsules are routinely employed by electron microscopists in tissue embedding procedures; growing monolayer cultures directly on the lids of inverted BEEM capsules presents the obvious advantage of maintaining cell to cell and cell to substratum conthaets with a minimum of stress and damage in the preparative steps for electron microscopy.