Waring G L, Mahowald A P
Cell. 1979 Mar;16(3):599-607. doi: 10.1016/0092-8674(79)90033-3.
The chorion of Drosophila melanogaster consists of proteins secreted by the follicular epithelium during late oogenesis. Petri, Wyman and Kafatos (1976) have described six major protein components of the Drosophila chorion and reported the synthesis of these proteins in vitro by mass-isolated egg chambers. We have used two-dimensional gel electrophoresis to identify approximately twenty components in highly purified chorion preparations. The synthesis patterns of these proteins in vivo were determined by isolating egg chambers of different developmental stages from flies injected with 14C amino acids. Chorion proteins constitute a large fraction of the protein synthesized by ovarian egg chambers in stages 12--14. The sizes and times of synthesis of the chorion proteins correlate closely with the production of poly(A)-containing RNAs by the follicle cells (Spradling and Mahowald, 1979).
黑腹果蝇的绒毛膜由卵泡上皮在卵子发生后期分泌的蛋白质组成。佩特里、怀曼和卡法托斯（1976年）描述了果蝇绒毛膜的六种主要蛋白质成分，并报告了通过大量分离的卵室在体外合成这些蛋白质的情况。我们使用二维凝胶电泳在高度纯化的绒毛膜制剂中鉴定出大约二十种成分。通过从注射了14C氨基酸的果蝇中分离不同发育阶段的卵室，确定了这些蛋白质在体内的合成模式。绒毛膜蛋白在12 - 14阶段占卵巢卵室合成蛋白质的很大一部分。绒毛膜蛋白的大小和合成时间与卵泡细胞产生含多聚腺苷酸（poly(A)）的RNA密切相关（斯普拉德林和马霍瓦尔德，1979年）。
