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s38 蛋白对黑腹果蝇卵巢蛋壳形态发生的不可或缺的贡献。

The indispensable contribution of s38 protein to ovarian-eggshell morphogenesis in Drosophila melanogaster.

机构信息

Section of Cell Biology and Biophysics, Department of Biology, School of Science, National and Kapodistrian University of Athens (NKUA), Athens, Greece.

Department of Cancer Biology, Medical School, University of Massachusetts, Worcester, Massachusetts (MA), USA.

出版信息

Sci Rep. 2018 Oct 31;8(1):16103. doi: 10.1038/s41598-018-34532-2.

DOI:10.1038/s41598-018-34532-2
PMID:30382186
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6208399/
Abstract

Drosophila chorion represents a remarkable model system for the in vivo study of complex extracellular-matrix architectures. For its organization and structure, s38 protein is considered as a component of major importance, since it is synthesized and secreted during early choriogenesis. However, there is no evidence that proves its essential, or redundant, role in chorion biogenesis. Hence, we show that targeted downregulation of s38 protein, specifically in the ovarian follicle-cell compartment, via employment of an RNAi-mediated strategy, causes generation of diverse dysmorphic phenotypes, regarding eggshell's regionally and radially specialized structures. Downregulation of s38 protein severely impairs fly's fertility and is unable to be compensated by the s36 homologous family member, thus unveiling s38 protein's essential contribution to chorion's assembly and function. Altogether, s38 acts as a key skeletal protein being critically implicated in the patterning establishment of a highly structured tripartite endochorion. Furthermore, it seems that s38 loss may sensitize choriogenesis to stochastic variation in its coordination and timing.

摘要

果蝇卵壳代表了一个用于研究复杂细胞外基质结构的重要模型系统。在卵壳的组织和结构中，s38 蛋白被认为是一个重要的组成部分，因为它在早期卵壳发生期间被合成和分泌。然而，目前尚无证据证明 s38 蛋白在卵壳发生中具有必需或冗余的作用。因此，我们通过 RNAi 介导的策略，特异性地下调卵巢滤泡细胞中 s38 蛋白的表达，发现其导致卵壳的区域性和径向特化结构产生多种畸形表型。s38 蛋白的下调严重损害了果蝇的生育能力，并且不能被同源家族成员 s36 所补偿，从而揭示了 s38 蛋白对卵壳组装和功能的重要贡献。总之，s38 作为一种关键的骨架蛋白，在高度结构化的三层内卵壳的模式建立中起着至关重要的作用。此外，s38 的缺失似乎使卵壳发生对其协调和时间的随机变化更加敏感。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/7d320a3d5385/41598_2018_34532_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/23882a8f48af/41598_2018_34532_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/cec0e4596cb2/41598_2018_34532_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/5b3e049f291b/41598_2018_34532_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/7b6365b489e4/41598_2018_34532_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/f64a77023da1/41598_2018_34532_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/0a069ae52770/41598_2018_34532_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/7d320a3d5385/41598_2018_34532_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/23882a8f48af/41598_2018_34532_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/cec0e4596cb2/41598_2018_34532_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/5b3e049f291b/41598_2018_34532_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/7b6365b489e4/41598_2018_34532_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/f64a77023da1/41598_2018_34532_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/0a069ae52770/41598_2018_34532_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94fb/6208399/7d320a3d5385/41598_2018_34532_Fig7_HTML.jpg

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