Wu N, Medina J C, Lee M L
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602-5700, USA.
J Chromatogr A. 2000 Sep 15;892(1-2):3-13. doi: 10.1016/s0021-9673(00)00152-7.
Packed capillary column solvating gas chromatography (SGC) and open tubular column gas chromatography (GC) were compared with respect to their potentials for fast separations. A recently introduced "universal" peak capacity equation was used to compare the performance of these two methods. The effects of various factors on peak capacity were investigated. Results demonstrate that retention factor and column efficiency are the main factors affecting peak capacity for fast separations. Packed columns produce both high retention factors and high selectivities. While high efficiencies and high peak capacities can be demonstrated by both techniques, open tubular column GC can surpass packed capillary column SGC in both measurements, except for the case of the analysis of simple mixtures in short analysis times, where retention factor and selectivity become important. Practical aspects such as pressure drop and sample capacity are compared for SGC and open tubular column GC. It was found that packed column SGC demonstrates higher sample capacities, but requires much higher column inlet pressures than open tubular column GC. A variety of mobile phases can be used for packed column SGC, which can provide high solvating power for large and polar compounds.
对填充毛细管柱溶剂化气相色谱法（SGC）和开管柱气相色谱法（GC）在快速分离方面的潜力进行了比较。使用最近引入的“通用”峰容量方程来比较这两种方法的性能。研究了各种因素对峰容量的影响。结果表明，保留因子和柱效是影响快速分离峰容量的主要因素。填充柱具有高保留因子和高选择性。虽然两种技术都能实现高效率和高的峰容量，但除了在短分析时间内分析简单混合物的情况（此时保留因子和选择性变得很重要）外，开管柱气相色谱法在这两个测量指标上都能超过填充毛细管柱溶剂化气相色谱法。比较了SGC和开管柱气相色谱法在压降和进样量等实际方面的情况。发现填充柱SGC具有更高的进样量，但比开管柱气相色谱法需要更高的柱入口压力。填充柱SGC可以使用多种流动相，这对于大分子和极性化合物能够提供高溶剂化能力。
