State Key Laboratory of Natural Medicines (China Pharmaceutical University), Nanjing, PR China.
Electrophoresis. 2013 May;34(9-10):1273-94. doi: 10.1002/elps.201200494.
Microemulsion electrokinetic chromatography (MEEKC) is a CE separation technique, which utilizes buffered microemulsions as the separation media. In the past two decades, MEEKC has blossomed into a powerful separation technique for the analysis of a wide range of compounds. Pseudostationary phase composition is so critical to successful resolution in EKC, and several variables could be optimized including surfactant/co-surfactant/oil type and concentration, buffer content, and pH value. Additionally, MEEKC coupled with online sample preconcentration approaches could significantly improve the detection sensitivity. This review comprehensively describes the development of MEEKC from the period 1991 to 2012. Areas covered include basic theory, microemulsion composition, improving resolution and enhancing sensitivity methods, detection techniques, and applications of MEEKC.
微乳液电动色谱（MEEKC）是一种 CE 分离技术，它利用缓冲微乳液作为分离介质。在过去的二十年中，MEEKC 已发展成为一种强大的分离技术，可用于分析多种化合物。在 EKC 中，准固定相组成对于成功分离至关重要，并且可以优化几个变量，包括表面活性剂/助表面活性剂/油的类型和浓度、缓冲液含量和 pH 值。此外，MEEKC 与在线样品预浓缩方法相结合，可以显著提高检测灵敏度。本综述全面描述了 1991 年至 2012 年期间 MEEKC 的发展情况。涵盖的领域包括基本理论、微乳液组成、提高分辨率和增强灵敏度的方法、检测技术以及 MEEKC 的应用。
