Brown L R, Bradbury J H, Austin K, Stewart P R
J Membr Biol. 1975 Oct 16;24(1):35-54. doi: 10.1007/BF01868614.
Proton magnetic resonance (PMR) and carbon-13 magnetic resonance (CMR) spectra of intact, unsonicated yeast and rat liver motochondria show differences which may be correlated with the composition of the membranes. High resolution PMR and CMR signals in intact yeast mitochondria have been assigned to regions of fluid lipid-lipid interaction on the basis of spectra of extracted lipid and protein, and the temperature dependence of NMR signals from the intact membrane. PMR spectra suggest that about 20% of total yeast phospholipid is in regions where both intramolecular fatty acid chain mobility and lateral diffusion of entire phospholipid molecules are possible. No such regions apear to exist in rat liver mitochondria. For both yeast and rat liver mitochondria, comparison of PMR and CMR spectra suggests that about 50% of phospholipid appears to be in regions where intramolecular fatty acid chain motion is considerable, but lateral diffusion is restricted. The remaining phospholipid appears to have little inter- or intramolecular mobility. Since NMR observation of lipid extracts from membranes indicates that phospholipid-sterol interactions do not account for the spectra of intact mitochondria, these effects are interpreted in terms of extensive lipid-protein interactions.
完整的、未超声处理的酵母和大鼠肝脏线粒体的质子磁共振（PMR）和碳-13磁共振（CMR）光谱显示出差异，这些差异可能与膜的组成相关。基于提取的脂质和蛋白质的光谱以及完整膜的NMR信号的温度依赖性，完整酵母线粒体中的高分辨率PMR和CMR信号已被归因于流体脂质-脂质相互作用区域。PMR光谱表明，酵母总磷脂中约20%处于分子内脂肪酸链可移动且整个磷脂分子可横向扩散的区域。大鼠肝脏线粒体中似乎不存在这样的区域。对于酵母和大鼠肝脏线粒体，PMR和CMR光谱的比较表明，约50%的磷脂似乎处于分子内脂肪酸链运动相当可观但横向扩散受限的区域。其余的磷脂似乎分子间或分子内流动性很小。由于对膜脂质提取物的NMR观察表明磷脂-甾醇相互作用不能解释完整线粒体的光谱，这些效应被解释为广泛的脂质-蛋白质相互作用。
