Austin K, Brown L R, Stewart P R
J Membr Biol. 1975 Oct 16;24(1):55-69. doi: 10.1007/BF01868615.
Promitochondrial membranes, prepared from Saccharomyces cerevisiae grown anaerobically under different conditions of lipid supplementation, have been examined by PMR spectroscopy. Promitochondria from cells cultured anaerobically in media containing both unsaturated fatty acid and sterol supplements, or containing unsaturated fatty acid alone, yield high resolution spectra similar to those which are characteristic of aerobic mitochondria. By contrast, promitochondrial membranes from cells grown only with sterol supplementation in order to deplete unsaturated fatty acid and total phospholipid content of the organelles, yielded PMR spectra which were very substantially broadened. These spectra are similar to those obtained with rat liver mitochondria. PMR spectra of promitochondria from each cell type dispersed in trifluoroacetic acid, or of extracted lipids or residual proteins similarly dispersed, were different only in detail. It appears, therefore, that in the native state membranes of unsaturated fatty acid-depleted promitochondria are structurally different from promitochondria of the other two cell types. The difference may be a consequence of altered lipid-to-protein ratios, and thus of changes in the extent of lipid domain formation in the membranes of these organelles.
从在不同脂质补充条件下厌氧培养的酿酒酵母中制备的前线粒体膜，已通过核磁共振波谱法进行了检测。在含有不饱和脂肪酸和固醇补充剂或仅含有不饱和脂肪酸的培养基中厌氧培养的细胞的前线粒体，产生的高分辨率光谱与需氧线粒体特有的光谱相似。相比之下，仅通过固醇补充来培养细胞，以耗尽细胞器中的不饱和脂肪酸和总磷脂含量，由此得到的前线粒体膜产生的核磁共振波谱明显变宽。这些光谱与从大鼠肝脏线粒体获得的光谱相似。分散在三氟乙酸中的每种细胞类型的前线粒体的核磁共振波谱，或类似分散的提取脂质或残留蛋白质的核磁共振波谱，仅在细节上有所不同。因此，似乎在天然状态下，不饱和脂肪酸耗尽的前线粒体的膜在结构上与其他两种细胞类型的前线粒体不同。这种差异可能是脂质与蛋白质比例改变的结果，进而也是这些细胞器膜中脂质结构域形成程度变化的结果。
