Borovanský J, Blasko M, Siracký J
2nd Department of Medical Chemistry and Biochemistry, 1st Faculty of Medicine of Charles University, Prague, Czech Republic.
Sb Lek. 2000;101(2):127-30.
"Leucine aminopeptidase" (LAP, aminopolypeptidase, EC 3.4.11) activity has been recommended and widely used as a histochemical marker for the identification of contaminating LAP-positive fibroblasts in pigment cell cultures. Using a sensitive biochemical assay with L-leucyl-p-nitroanilide as a substrate we demonstrated that in vitro melanoma cells also exhibit LAP activity. Our comparison of four melanoma cell lines with four fibroblast lines showed that the differences in the enzyme activity were not qualitative but only quantitative. For this reason the specific antibodies, karyological analysis and electron microscopy are recommended as more reliable means in distinguishing fibroblasts from poorly differentiated pigment cells than the LAP-cytochemistry.
“亮氨酸氨肽酶”（LAP，氨基多肽酶，EC 3.4.11）活性已被推荐并广泛用作一种组织化学标志物，用于鉴定色素细胞培养物中污染的LAP阳性成纤维细胞。我们使用以L-亮氨酰对硝基苯胺为底物的灵敏生化测定法证明，体外培养的黑素瘤细胞也表现出LAP活性。我们将四种黑素瘤细胞系与四种成纤维细胞系进行比较，结果表明酶活性的差异并非质的不同，而只是量的差异。因此，比起LAP细胞化学法，推荐使用特异性抗体、染色体分析和电子显微镜检查作为区分成纤维细胞与低分化色素细胞的更可靠方法。
