Delcroix M, Libert A, Lejeune F J
Anticancer Res. 1986 Nov-Dec;6(6):1369-75.
Although it is well known that fibroblasts can overgrow melanoma cells in culture during early passaging, some investigators have claimed recently that these fibroblast-like cells are actually melanocytes de-differentiated in vitro. In order to test these claims, we examined 27 melanoma-derived cell lines using anti-melanoma and anti-fibroblast monoclonal antibodies, the dioxyphenylalanine cytochemical reaction specific for melanocytes, the leucine aminopeptidase cytochemical reaction for fibroblast cells, and chromosomal analysis. All these assays - which discriminate melanomas from fibroblasts - confirmed our initial classification by morphology, namely that thirteen of the lines were melanocytic and twelve were fibroblastic, while the remaining two were a mixture of the two cell types. There was no indication of a mixed phenotype which would have been the result of some transaction in the state of differentiation. Our results suggest that the 27 cell lines and cultures are composed of pure melanocytic populations, or are fibroblasts as a result of fibroblast overgrowth. The two cultures containing a mixed cell population are those in which one cell type has not overtaken the other. There is no evidence to support the idea that some true melanoma cultures have the morphology of fibroblast-like cells.
尽管众所周知，在早期传代培养过程中，成纤维细胞能够在体外生长超过黑色素瘤细胞，但最近一些研究人员声称，这些成纤维细胞样细胞实际上是在体外去分化的黑色素细胞。为了验证这些说法，我们使用抗黑色素瘤和抗成纤维细胞单克隆抗体、黑色素细胞特异性的二羟苯丙氨酸细胞化学反应、成纤维细胞的亮氨酸氨肽酶细胞化学反应以及染色体分析，对27个黑色素瘤来源的细胞系进行了检测。所有这些能够区分黑色素瘤和成纤维细胞的检测方法，都证实了我们最初根据形态学进行的分类，即其中13个细胞系是黑色素细胞性的，12个是成纤维细胞性的，而其余两个是两种细胞类型的混合物。没有迹象表明存在混合表型，而混合表型本应是分化状态发生某种转变的结果。我们的结果表明，这27个细胞系和培养物要么由纯黑色素细胞群体组成，要么是成纤维细胞过度生长导致的成纤维细胞。含有混合细胞群体的两种培养物是其中一种细胞类型尚未超过另一种细胞类型的培养物。没有证据支持某些真正的黑色素瘤培养物具有成纤维细胞样细胞形态这一观点。
