Obata T, Tomaru K, Nagakura T, Izumi Y, Kawamoto T
Department of Molecular Cell Biology, Institute of DNA Medicine, Jikei University School of Medicine, Minato, Tokyo, Japan.
J Chromatogr B Biomed Sci Appl. 2000 Sep 1;746(1):11-5. doi: 10.1016/s0378-4347(00)00182-1.
Isoprostane (8-epi-prostaglandin F2alpha) is synthesized non-enzymatically from arachidonate and active oxygen. We examined the relationship of smoking and excretion of isoprostane in urine with gas chromatography-mass spectrometry selected ion monitoring assay and the stable isotope dilution method. Urine isoprostane concentrations were significantly higher in smokers (n=81, 605.24+/-59.01 ng/mg creatinine) than in non-smokers (n=39, 424.07+/-70.37 ng/mg creatinine), but concentrations in ex-smokers (n=21, 487.27+/-98.48 ng/mg creatinine) did not differ significantly from those in the other groups. In smokers, age, the duration of smoking, and the number of cigarettes per day were not correlated with urine isoprostane concentrations. However, urine isoprostane concentrations were negatively correlated with time since quitting in ex-smokers and with age in non-smokers. These results indicate that smoking increases isoprostane concentration in urine and suggest that smoking causes lipid peroxidation by oxidant stress.
异前列腺素（8-表-前列腺素F2α）是由花生四烯酸和活性氧非酶促合成的。我们采用气相色谱-质谱选择离子监测法和稳定同位素稀释法研究了吸烟与尿中异前列腺素排泄之间的关系。吸烟者（n = 81，605.24±59.01 ng/mg肌酐）的尿异前列腺素浓度显著高于非吸烟者（n = 39，424.07±70.37 ng/mg肌酐），但戒烟者（n = 21，487.27±98.48 ng/mg肌酐）的浓度与其他组无显著差异。在吸烟者中，年龄、吸烟持续时间和每日吸烟量与尿异前列腺素浓度无关。然而，戒烟者的尿异前列腺素浓度与戒烟时间呈负相关，非吸烟者的尿异前列腺素浓度与年龄呈负相关。这些结果表明吸烟会增加尿中异前列腺素浓度，并提示吸烟通过氧化应激导致脂质过氧化。
