Gergely A, Horváth P, Noszál B
Institute of Pharmaceutical Chemistry, Semmelweis University, Budapest, Hungary.
J Chromatogr Sci. 2000 Oct;38(10):425-9. doi: 10.1093/chromsci/38.10.425.
Composite chromatographic peaks are deconvoluted by a method that uses ratio formation from signals of simultaneous double detection. The method is generally suitable if two detector signals can simultaneously be acquired and their uses do not need any a priori assumption or mathematical shape analysis. A simple deduction makes the compound- and detector-specific intensive parameters explicit, which allows for the digital construction of directly invisible component peaks. The simultaneous double detection is shown to be superior to coupled detectors, sequentially fixed chromatograms, and subsequently synchronized peaks. The combination of circular dichroism and ultraviolet (UV) detection is shown to be especially advantageous in the analysis of enantiomers for which the other ratio-forming peak-deconvolution techniques have inherently been insensitive. The double chiroptical UV detection can be of further use to decompose overlapping peaks of nonenantiomeric compounds that are highly similar. The capacity of the method is exemplified by the analysis of morphine alkaloids, steroid oximes, and synthetic heterocycles.
复合色谱峰通过一种利用同时双检测信号形成比率的方法进行解卷积。如果能够同时采集两个检测器信号，并且其使用不需要任何先验假设或数学形状分析，那么该方法通常是适用的。一个简单的推导可以明确化合物和检测器特定的强度参数，这使得可以数字构建直接不可见的组分峰。结果表明，同时双检测优于联用检测器、顺序固定色谱图以及随后同步的峰。圆二色性和紫外（UV）检测的结合在对映体分析中显示出特别的优势，因为其他形成比率的峰解卷积技术对此本质上不敏感。双旋光紫外检测可进一步用于分解高度相似的非对映体化合物的重叠峰。该方法的能力通过吗啡生物碱、甾体肟和合成杂环的分析得到了例证。
