Daniels J T, Hatfield P G, Burgess D E, Kottt R W, Bowman J G
Department of Animal and Range Sciences, Montana State University, Bozeman 59717-2900, USA.
J Anim Sci. 2000 Oct;78(10):2731-6. doi: 10.2527/2000.78102731x.
Fifty-two Targhee twin-bearing ewes were used in a factorial arrangement of treatments to investigate the role of supplemental vitamin E (vit E); 0 (NE) vs 400 IU of vit E x ewe x (-1)d(-1) (E) and parainfluenza type 3 (PI3) vaccination; none (NP) vs PI3 vaccination (P) in immune function. Parainfluenza type 3 vaccination was used to evoke an immune response. Ewes receiving PI3 were vaccinated at 49 and 21 d before the expected lambing date. Ewes receiving vit E were orally dosed daily, 32 to 0 d before lambing. Blood was collected from ewes at the time of the initial PI3 vaccination and 4 h postpartum. Blood was collected from lambs (n = 104) at 3 d postpartum. Ewe and lamb sera were analyzed for anti-PI3 antibody titers, immunoglobulin G (IgG) titers, and vit E concentrations. Colostrum was collected 4 h postpartum and analyzed for IgG. The model for ewe and lamb analysis included the main effects of vit E and PI3, sex (lambs model only), and their interactions. No interactions were detected (P > 0.20) for any ewe or lamb variables. Serum anti-PI3 titers were greater (P < 0.01) in P ewes and their lambs than NP ewes and their lambs. Serum vit E concentrations were greater (P < 0.01) in E ewes and their lambs than NE ewes and their lambs. Colostral IgG titers and serum anti-PI3 titers did not differ (P > 0.20) between E and NE ewes. Serum IgG titers in E ewes and their lambs did not differ (P > 0.15) from IgG titers in NE ewes and their lambs. Lamb anti-PI3 titers did not differ (P = 0.76) between lambs reared by E and NE ewes. These results indicate that, although supplemental vit E to the ewe increased lamb serum vit E concentration, it had no effect on measures used in this study to assess humoral immunity in the ewe or passive immunity to the lamb.
52只携带双胞胎的塔吉母羊被用于析因处理安排，以研究补充维生素E（维生素E）的作用；每只母羊每天补充0（NE）或400国际单位维生素E×（-1）天（E）以及接种3型副流感病毒（PI3）疫苗；未接种（NP）与接种PI3疫苗（P）对免疫功能的影响。使用3型副流感病毒疫苗来引发免疫反应。接受PI3疫苗接种的母羊在预计产羔日期前49天和21天进行接种。接受维生素E的母羊在产羔前32天至0天每天口服给药。在初次接种PI3疫苗时和产后4小时从母羊采集血液。在产后3天从羔羊（n = 104）采集血液。分析母羊和羔羊血清中的抗PI3抗体滴度、免疫球蛋白G（IgG）滴度以及维生素E浓度。在产后4小时采集初乳并分析其中的IgG。母羊和羔羊分析模型包括维生素E和PI3的主效应、性别（仅羔羊模型）及其相互作用。对于任何母羊或羔羊变量均未检测到相互作用（P > 0.20）。接种PI3疫苗的母羊及其羔羊的血清抗PI3滴度高于未接种PI3疫苗的母羊及其羔羊（P < 0.01）。补充维生素E的母羊及其羔羊的血清维生素E浓度高于未补充维生素E的母羊及其羔羊（P < 0.01）。补充维生素E的母羊和未补充维生素E的母羊的初乳IgG滴度和血清抗PI3滴度没有差异（P > 0.20）。补充维生素E的母羊及其羔羊的血清IgG滴度与未补充维生素E的母羊及其羔羊的IgG滴度没有差异（P > 0.15）。由补充维生素E的母羊和未补充维生素E的母羊饲养的羔羊的抗PI3滴度没有差异（P = 0.76）。这些结果表明，虽然给母羊补充维生素E会提高羔羊血清维生素E浓度，但对本研究中用于评估母羊体液免疫或羔羊被动免疫的指标没有影响。
