噬菌体T7早期信使核糖核酸的化学稳定性
Chemical stability of bacteriophage T7 early mRNA.
作者信息
Yamada Y, Whitaker P A, Nakada D
出版信息
J Virol. 1975 Dec;16(6):1683-7. doi: 10.1128/JVI.16.6.1683-1687.1975.
Abstract
T7 early mRNA produced by a gene 1 amber mutant phage, T7 am27, is chemically stable interms of acid insolubility and T7 DNA hybridizability. However, the messenger activity of individual T7 early mRNA species, transcripts of gene 1, gene 0.7, and gene 1.3, decay with a half-life of about 6.5 min at 30 C. An extensive secondary structure is present in all T7 early mRNA species and is probably responsible for the chemical stability of the RNAs after the loss of functional activity. It is unlikely that ribosomes protect T7 early mRNA from nucleolytic degradation.
摘要
由基因1琥珀突变噬菌体T7 am27产生的T7早期mRNA，在酸不溶性和T7 DNA杂交性方面化学性质稳定。然而，基因1、基因0.7和基因1.3的单个T7早期mRNA种类的信使活性在30℃下以约6.5分钟的半衰期衰减。所有T7早期mRNA种类中都存在广泛的二级结构，这可能是RNA功能活性丧失后化学稳定性的原因。核糖体不太可能保护T7早期mRNA免受核酸酶降解。
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