Ademuyiwa O, Elsenhans B
Walther-Straub-lnstitut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität München, Germany.
Biol Trace Elem Res. 2000 Apr;74(1):81-92. doi: 10.1385/BTER:74:1:81.
Oral administration of inorganic arsenic has been shown to lead to an accumulation of copper in the kidneys of rats and guinea pigs. However, nothing is known about the characteristics and mechanisms of this organ-specific renal copper accumulation. Many heavy metals accumulate in the kidney, either after environmental or occupational exposure. An additional accumulation of any other trace metals, even essential ones, may therefore be critical for that organ. This prompted us to follow the course of the renal copper accumulation. Rats were given daily subcutaneous doses of sodium arsenite for 12 d. Each second day, three rats were killed by exsanguination and the liver, kidneys, and blood removed and analysed for As, Cu, and other trace elements by atomic emission spectrometry. Results indicate that arsenic and copper accumulate in the kidney cortex synchroneously over time. Arsenic also accumulated in the liver and red blood cells (RBC). Copper levels in the RBC and liver as well as copper excretion into the urine were unaffected. After terminating arsenite administration, there was a slow decline in tissue levels of both arsenic and copper, a phenomenon which was parallel for both metals. Because the copper level in the liver was not affected, it is concluded from this study that renal processes and not hepatic or biliary mechanisms might be responsible for the renal copper accumulation. Furthermore, the strong linear correlation (r = 0.85) between arsenic and copper levels in the kidney during and after arsenite administration suggests a functional relationship between arsenic and copper with respect to their retention in the kidney.
已证实口服无机砷会导致大鼠和豚鼠肾脏中铜的蓄积。然而，关于这种器官特异性肾脏铜蓄积的特征和机制尚不清楚。许多重金属在环境或职业暴露后会在肾脏中蓄积。因此，任何其他微量元素（即使是必需元素）的额外蓄积可能对该器官至关重要。这促使我们追踪肾脏铜蓄积的过程。给大鼠每日皮下注射亚砷酸钠，持续12天。每隔一天，处死三只大鼠，通过放血法取其肝脏、肾脏和血液，并用原子发射光谱法分析其中的砷、铜和其他微量元素。结果表明，随着时间的推移，砷和铜在肾皮质中同步蓄积。砷也在肝脏和红细胞（RBC）中蓄积。红细胞和肝脏中的铜水平以及尿铜排泄均未受影响。停止注射亚砷酸钠后，砷和铜的组织水平均缓慢下降，两种金属的这种现象是平行的。由于肝脏中的铜水平未受影响，本研究得出结论，肾脏过程而非肝脏或胆汁机制可能是肾脏铜蓄积的原因。此外，在注射亚砷酸钠期间及之后，肾脏中砷和铜水平之间存在很强的线性相关性（r = 0.85），这表明砷和铜在肾脏潴留方面存在功能关系。
