Flores G V, Duan H, Yan H, Nagaraj R, Fu W, Zou Y, Noll M, Banerjee U
Department of Molecular, Cell, and Developmental Biology and Molecular Biology Institute, University of California at Los Angeles 90095, USA.
Cell. 2000 Sep 29;103(1):75-85. doi: 10.1016/s0092-8674(00)00106-9.
How multifunctional signals combine to specify unique cell fates during pattern formation is not well understood. Here, we demonstrate that together with the transcription factor Lozenge, the nuclear effectors of the EGFR and Notch signaling pathways directly regulate D-Pax2 transcription in cone cells of the Drosophila eye disc. Moreover, the specificity of D-Pax2 expression can be altered upon genetic manipulation of these inputs. Thus, a relatively small number of temporally and spatially controlled signals received by a set of pluripotent cells can create the unique combinations of activated transcription factors required to regulate target genes and ultimately specify distinct cell fates within this group. We expect that similar mechanisms may specify pattern formation in vertebrate developmental systems that involve intercellular communication.
在模式形成过程中，多功能信号如何组合以指定独特的细胞命运，目前尚不清楚。在这里，我们证明，与转录因子菱形（Lozenge）一起，表皮生长因子受体（EGFR）和Notch信号通路的核效应器直接调节果蝇眼盘视锥细胞中D-Pax2的转录。此外，对这些输入进行基因操作后，D-Pax2表达的特异性会发生改变。因此，一组多能细胞接收到的相对少量的时空控制信号，可以产生调节靶基因所需的激活转录因子的独特组合，并最终在该组细胞中指定不同的细胞命运。我们预计，类似的机制可能在涉及细胞间通讯的脊椎动物发育系统中指定模式形成。
