Slejkovec Z, van Elteren J T, Woroniecka U D, Kroon K J, Falnoga I, Byrne A R
Interfaculty Reactor Institute, Delft University of Technology, The Netherlands.
Biol Trace Elem Res. 2000 Summer;75(1-3):139-55. doi: 10.1385/BTER:75:1-3:139.
Using various chromatographic techniques (size exclusion, anion exchange, and cation exchange) combined with several detectors (neutron activation analysis and atomic fluorescence spectrometry), an attempt was made to characterize selenium compounds in some edible, selenium-accumulating mushrooms (Albatrellus pes-caprae and Boletus edulis). The mushrooms contained mostly low-molecular-weight (6 kDa) selenium compounds. After proteolysis, only a small fraction of the extractable selenium could be identified as selenite (3.0-9.2%, Albatrellus pes-caprae), selenocystine (minor, Albatrellus pes-caprae; 7.5%, Boletus edulis), or selenomethionine (1.0%, Boletus edulis), leaving the form of the bulk still to be elucidated.
运用多种色谱技术（尺寸排阻色谱、阴离子交换色谱和阳离子交换色谱）并结合几种检测器（中子活化分析和原子荧光光谱法），对一些可食用的、能富集硒的蘑菇（阿尔巴瑞蘑和美味牛肝菌）中的硒化合物进行了表征。这些蘑菇中大多含有低分子量（6 kDa）的硒化合物。蛋白水解后，可提取的硒中只有一小部分能被鉴定为亚硒酸盐（3.0 - 9.2%，阿尔巴瑞蘑）、硒代胱氨酸（少量，阿尔巴瑞蘑；7.5%，美味牛肝菌）或硒代蛋氨酸（1.0%，美味牛肝菌），大部分硒的存在形式仍有待阐明。
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