巴西坚果（Bertholletia excelsa）蛋白质组分中硒分布的研究。

Study of selenium distribution in the protein fractions of the Brazil nut, Bertholletia excelsa.

作者信息

Chunhieng Thavarith, Pétritis Konstantinos, Elfakir Claire, Brochier José, Goli Thierry, Montet Didier

机构信息

Cirad-Amis, PAA, TA 40/16, 73 rue J.-F. Breton, 34398 Montpellier Cedex 5, France.

出版信息

J Agric Food Chem. 2004 Jun 30;52(13):4318-22. doi: 10.1021/jf049643e.

DOI:10.1021/jf049643e

PMID:15212486

Abstract

The high selenium content of the Brazil nut, Bertholletia excelsa, makes this seed a healthy food qualified as an antiradical protector. The studied nut contained 126 ppm of selenium. Selenium was found to be distributed in the nut protein fractions. The water-extracted fraction, which represented 17.7% of the cake protein, was the richest in selenium with 153 ppm. Analysis by HPLC-MS showed that selenium was linked by a covalent bond to two amino acids to form selenomethionine and selenocystine. The selenomethionine represented a little less than 1% of the total amount of methionine.

摘要

巴西坚果（Bertholletia excelsa）中硒含量很高，这使得这种种子成为一种合格的具有抗自由基保护作用的健康食品。所研究的坚果含硒量为126 ppm。发现硒分布在坚果的蛋白质组分中。水提取组分占饼粕蛋白的17.7%，是硒含量最丰富的组分，含硒量为153 ppm。通过HPLC-MS分析表明，硒通过共价键与两种氨基酸相连，形成硒代蛋氨酸和硒代胱氨酸。硒代蛋氨酸占蛋氨酸总量的比例略低于1%。