Hosokawa M, Shimatani T, Kanada T, Inoue Y, Takahashi K
Faculty of Fisheries, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
J Agric Food Chem. 2000 Oct;48(10):4550-4. doi: 10.1021/jf991186s.
Phospholipase D (PLD)-mediated transphosphatidylation of squid skin lecithin with L-serine was examined to prepare docosahexaenoic acid-containing phosphatidylserine (DHA-PS). When a biphasic system with organic solvent and 0.2 M acetate buffer (pH 5.5) was used, PS synthesis was significantly affected by the amount of 3.4 M L-serine-containing acetate buffer. L-Serine concentration in the acetate buffer and choice of organic solvent were also crucial. In a typical reaction with 0.8 unit of PLD (Streptomyces sp.), 2.5 mL of ethyl acetate substrate solution containing 30 mg of squid skin lecithin in combination with 3 mL of 3.4 M L-serine-containing 0.2 M acetate buffer (pH 5.5), PS content in the recovered phospholipid fraction increased to 43.1% after 24 h. DHA composed 37.6% of fatty acids in the converted PS. This was the same DHA level as in the substrate. Phosphatidylcholine (squid skin PC, DHA 44.2%) in the squid skin lecithin was more effectively converted to PS than phosphatidylethanolamine.
研究了磷脂酶D（PLD）介导的鱿鱼皮卵磷脂与L-丝氨酸的转磷脂酰化反应，以制备含二十二碳六烯酸的磷脂酰丝氨酸（DHA-PS）。当使用有机溶剂和0.2 M醋酸盐缓冲液（pH 5.5）的双相体系时，含3.4 M L-丝氨酸的醋酸盐缓冲液的量对PS合成有显著影响。醋酸盐缓冲液中L-丝氨酸的浓度和有机溶剂的选择也至关重要。在典型反应中，使用0.8单位的PLD（链霉菌属），2.5 mL含有30 mg鱿鱼皮卵磷脂的乙酸乙酯底物溶液与3 mL含3.4 M L-丝氨酸的0.2 M醋酸盐缓冲液（pH 5.5）混合，24小时后回收的磷脂部分中PS含量增加到43.1%。DHA在转化后的PS中占脂肪酸的37.6%。这与底物中的DHA水平相同。鱿鱼皮卵磷脂中的磷脂酰胆碱（鱿鱼皮PC，DHA 44.2%）比磷脂酰乙醇胺更有效地转化为PS。
