Damiens C, Fortun Y, Charrier C, Heymann D, Padrines M
Laboratoire de Physiopathologie de la Résorption Osseuse, EE 99-01, Faculté de Médecine, 1 rue Gaston Veil, Nantes, 44035, France.
Cytokine. 2000 Nov;12(11):1727-31. doi: 10.1006/cyto.2000.0765.
This study investigated the ability of normal human osteoblasts (hOb) and osteogenic sarcoma cells (MG-63 and SaOS2) to produce gelatinases and undergo modulation by interleukin 1beta (IL-1beta), interleukin 6 (IL-6), oncostatin M (OSM), leukaemia inhibitory factor (LIF), growth hormone (GH) and insulin-like growth factor-I (IGF-I). Gelatinase activities were determined by zymogaphy, and a quantitative analysis was performed by ELISA. The MMP-2 activities of the three cell lines were significantly increased in the presence of IL-1beta and IL-6, but no modulation of MMP-2 activities was observed in the presence of OSM, LIF and GH. IGF-I increased the activity released by SaOS2 and hOb, but no modulation was detectable in MG-63 cell conditioned medium. An upmodulation of pro-MMP-2 secretion by SaOS2 and hOb was observed for all soluble factors used, whereas an upmodulation of pro-MMP-2 secretion by MG-63 was observed only in the presence of IL-1beta, IL-6 and IGF-I. Thus, osteoblastic cells modulated by cytokines can be involved in bone resorption as a result of the protease activities released.
本研究调查了正常人成骨细胞（hOb）和成骨肉瘤细胞（MG-63和SaOS2）产生明胶酶以及受白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、制瘤素M（OSM）、白血病抑制因子（LIF）、生长激素（GH）和胰岛素样生长因子-I（IGF-I）调节的能力。通过酶谱法测定明胶酶活性，并通过酶联免疫吸附测定法进行定量分析。在IL-1β和IL-6存在的情况下，三种细胞系的基质金属蛋白酶-2（MMP-2）活性显著增加，但在OSM、LIF和GH存在的情况下未观察到MMP-2活性的调节。IGF-I增加了SaOS2和hOb释放的活性，但在MG-63细胞条件培养基中未检测到调节作用。对于所有使用的可溶性因子，观察到SaOS2和hOb的前MMP-2分泌上调，而仅在IL-1β、IL-6和IGF-I存在的情况下观察到MG-63的前MMP-2分泌上调。因此，细胞因子调节的成骨细胞可能由于释放的蛋白酶活性而参与骨吸收。
