de Lana M, da Silviera Pinto A, Bastrenta B, Barnabé C, Noël S, Tibayrenc M
Centre d'Etudes sur le Polymorphisme des Microorganismes (CEPM), Unité Mixte de Recherche no. 9926 Centre National de la Recherche Scientifique (CNRS)/Institut de Recherche pour le Développement (IRD), B.P. 5045, 34032 Montpellier Cedex 1, France.
Exp Parasitol. 2000 Oct;96(2):61-6. doi: 10.1006/expr.2000.4552.
Eight Trypanosoma cruzi stocks pertaining to the clonal genotypes 19/20, 32, and 39 have been characterized for three experimental parameters of infectivity in Balb/c mice: (i) percentage of mice with a patent parasitemia (% MPP), (ii) maximum parasitemia (MP), and (iii) percentage of mice with positive hemoculture (% MPH). By order of decreasing values, the values recorded for the clonal genotypes ranked as follows: 19/20, 32, and 39, except for the % MPP parameter, for which 19/20 and 32 were not statistically different. The rate of successful reisolation after infection in mice, analyzed by multilocus enzyme electrophoresis and random amplified polymorphic DNA typing, was statistically different according to the clonal genotype and was different for uniclonal infections and for mixed infections by two different clonal genotypes. These results confirm that T. cruzi clonal genotypes differ significantly in their infectivity in mice.
对属于克隆基因型19/20、32和39的八株克氏锥虫进行了表征，涉及Balb/c小鼠感染性的三个实验参数：（i）出现显性寄生虫血症的小鼠百分比（%MPP），（ii）最大寄生虫血症（MP），以及（iii）血培养呈阳性的小鼠百分比（%MPH）。按数值递减顺序排列，克隆基因型记录的值如下：19/20、32和39，但%MPP参数除外，对于该参数，19/20和32在统计学上无差异。通过多位点酶电泳和随机扩增多态性DNA分型分析，小鼠感染后成功再分离的比率根据克隆基因型在统计学上存在差异，并且单克隆感染和两种不同克隆基因型的混合感染也有所不同。这些结果证实，克氏锥虫克隆基因型在小鼠中的感染性存在显著差异。
