Miyamoto Claudia Tiemi, Gomes Mônica Lúcia, Marangon Aline Vansan, de Araújo Silvana Marques, Bahia Maria Terezinha, Martins-Filho Olindo Assis, de Lana Marta, de Ornelas Toledo Max Jean
Departamento de Análises Clínicas, Centro de Ciencias da Saude, Universidade Estadual de Maringá, Av. Colombo, 87020-900 Maringá, Paraná, Brazil.
Exp Parasitol. 2008 Sep;120(1):45-9. doi: 10.1016/j.exppara.2008.04.018. Epub 2008 May 1.
The capacity of the polymerase chain reaction (PCR) to detect the DNA of Trypanosoma cruzi was evaluated in 90 blood samples from BALB/c mice infected with T. cruzi cloned stocks of genotypes 19 and 20 (T. cruzi I) and 39 and 32 (T. cruzi II), and treated with benznidazole. The results from the fresh blood examination, hemoculture, and ELISA allowed to group the treated animals into: cured (TC), dissociated (DIS) and non-cured (NC). The PCR detected T. cruzi DNA in 50.9%, 58.3% and 100.0% of the samples from TC, DIS and NC mice, respectively. These DNA possibly derives from live T. cruzi or from recently lysed parasites, suggests that these animals are in fact not cured. The difference between the PCR results and results obtained using other techniques was statistically significant and independent of the parasite genotype. The PCR described has therefore potential to be used in cure control of treated patients.
评估了聚合酶链反应（PCR）检测克氏锥虫DNA的能力，该检测针对90份来自感染了基因型19和20（克氏锥虫I型）以及39和32（克氏锥虫II型）的克氏锥虫克隆株并接受苯硝唑治疗的BALB/c小鼠的血液样本。新鲜血液检查、血液培养和酶联免疫吸附测定（ELISA）的结果使接受治疗的动物被分为：治愈（TC）、解离（DIS）和未治愈（NC）。PCR分别在来自TC、DIS和NC小鼠的样本中检测到克氏锥虫DNA的比例为50.9%、58.3%和100.0%。这些DNA可能来源于活的克氏锥虫或最近裂解的寄生虫，这表明这些动物实际上并未治愈。PCR结果与使用其他技术获得的结果之间的差异具有统计学意义，且与寄生虫基因型无关。因此，所描述的PCR有潜力用于治疗患者的治愈控制。
