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16S rRNA（鸟嘌呤-N2）甲基转移酶的系统基因组分析表明存在新的家族成员，并揭示了高度保守的基序以及与其他核酸氨基甲基转移酶相似的结构域结构。

Phylogenomic analysis of 16S rRNA:(guanine-N2) methyltransferases suggests new family members and reveals highly conserved motifs and a domain structure similar to other nucleic acid amino-methyltransferases.

作者信息

Bujnicki J M

机构信息

Bioinformatics Unit, International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland.

出版信息

FASEB J. 2000 Nov;14(14):2365-8. doi: 10.1096/fj.00-0076com.

DOI:10.1096/fj.00-0076com
PMID:11053259
Abstract

The sequences of known Escherichia coli 16S rRNA:m2G1207 methyltransferase (MTase) RsmC and hypothetical 16S rRNA:m2G966 MTase encoded by the ygjo open reading frame were used to carry out a database search of other putative m2G-generating enzymes in finished and unfinished genomic sequences. Sequence comparison and phylogenetic analysis of 21 close homologs of RsmC and YgjO revealed the presence of the third paralogous lineage in E. coli and other gamma-Proteobacteria, which might correspond to the subfamily of MTases specific for G1516 in 16S rRNA. In addition, the comparative sequence analysis supported by sequence/structure threading suggests that rRNA:m2G MTases are very closely related to RNA and DNA:m6A MTases and that these two enzyme families share common architecture of the active site and presumably a similar mechanism of methyl group transfer onto the exocyclic amino group of their target bases.

摘要

已知的大肠杆菌16S rRNA:m2G1207甲基转移酶（MTase）RsmC以及由ygjo开放阅读框编码的假定16S rRNA:m2G966 MTase的序列，被用于在已完成和未完成的基因组序列中对其他推定的产生m2G的酶进行数据库搜索。对RsmC和YgjO的21个紧密同源物进行序列比较和系统发育分析，揭示了大肠杆菌和其他γ-变形菌中第三个旁系同源谱系的存在，这可能对应于16S rRNA中对G1516特异的MTase亚家族。此外，由序列/结构穿线支持的比较序列分析表明，rRNA:m2G MTases与RNA和DNA:m6A MTases密切相关，并且这两个酶家族共享活性位点的共同结构，推测其甲基转移到靶碱基环外氨基上的机制相似。

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