Song E Y, Kang S J, Lee Y J, Park M H
Department of Clinical Pathology, Seoul National University College of Medicine, Seoul, South Korea.
Hum Immunol. 2000 Sep;61(9):937-41. doi: 10.1016/s0198-8859(00)00155-5.
There are considerable racial differences in the distribution of HLA-DR2-associated DRB1 and DRB5 alleles and the characteristics of linkage disequilibrium between these alleles. In this study, the frequencies of DR2-associated DRB1 and DRB5 alleles and related haplotypes were analyzed in 186 DR2-positive individuals out of 800 normal Koreans registered for unrelated bone marrow donors. HLA class I antigen typing was performed by the serological method and DRB1 and DRB5 genotyping by the PCR-single strand conformational polymorphism method. Only 3 alleles were detected for DR2-associated DRB1 and DRB5 genes, respectively: DRB1()1501 (gene frequency 8.0%), ()1502 (3.2%), ()1602 (0.9%); DRB5()0101 (8.0%), ()0102 (3.2%), and ()0202 (0.9%). DRB1-DRB5 haplotype analysis showed an exclusive association between these alleles: DRB11501-DRB50101 (haplotype frequency 8.0%), DRB1()1502-DRB5()0102 (3.2%), and DRB1()1602-DRB5()0202 (0.9%). The 5 most common DR2-associated A-B-DRB1 haplotypes occurring at frequencies of > or = 0.5% were A24-B52-DRB1()1502 (1.8%), A2-B62-DRB1()1501, A2-B54-DRB1()1501, A26-B61-DRB1()1501, and A24-B51-DRB1(*)1501. The remarkable homogeneity in the haplotypic associations between DR2-associated DRB1 and DRB5 alleles in Koreans would be advantageous for organ transplantation compared with other ethnic groups showing considerable heterogeneity in the distribution of DRB1-DRB5 haplotypes.
HLA-DR2相关的DRB1和DRB5等位基因的分布以及这些等位基因之间的连锁不平衡特征存在相当大的种族差异。在本研究中，对800名登记为非亲属骨髓供者的正常韩国人中的186名DR2阳性个体进行了DR2相关的DRB1和DRB5等位基因及相关单倍型频率分析。采用血清学方法进行HLA I类抗原分型，采用PCR-单链构象多态性方法进行DRB1和DRB5基因分型。DR2相关的DRB1和DRB5基因分别仅检测到3个等位基因：DRB1（）1501（基因频率8.0%）、（）1502（3.2%）、（）1602（0.9%）；DRB5（）0101（8.0%）、（）0102（3.2%）和（）0202（0.9%）。DRB1-DRB5单倍型分析显示这些等位基因之间存在排他性关联：DRB11501-DRB50101（单倍型频率8.0%）、DRB1（）1502-DRB5（）0102（3.2%）和DRB1（）1602-DRB5（）0202（0.9%）。出现频率≥0.5%的5种最常见的DR2相关A-B-DRB1单倍型为A24-B52-DRB1（）1502（1.8%）、A2-B62-DRB1（）1501、A2-B54-DRB1（）1501、A26-B61-DRB1（）1501和A24-B51-DRB1（*）1501。与其他在DRB1-DRB5单倍型分布上表现出相当大异质性的种族群体相比，韩国人中DR2相关的DRB1和DRB5等位基因之间单倍型关联的显著同质性对器官移植将是有利的。
