Chan F L, Choi H L, Chen Z Y, Chan P S, Huang Y
Department of Anatomy, Basic Medical Sciences Building, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China.
Cancer Lett. 2000 Nov 28;160(2):219-28. doi: 10.1016/s0304-3835(00)00591-7.
The flavonoid baicalin (baicalein 7-D-beta-glucuronate), isolated from the dried root of Scutellaria baicalensis Georgi (Huang Qin), is widely used in the traditional Chinese herbal medicine for its anti-inflammatory, anti-pyretic and anti-hypersensitivity effects. In the present study, we investigated the in vitro effects of baicalin on the growth, viability, and induction of apoptosis in several human prostate cancer cell lines, including DU145, PC-3, LNCaP and CA-HPV-10. The cell viability after treating with baicalin for 2-4 days was quantified by a colorimetric 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-s ulfophenyl)- 2H-tetrazolium (MTS) assay. The results showed that baicalin could inhibit the proliferation of prostate cancer cells. The responses to baicalin were different among different cell lines, with DU145 cells being the most sensitive and LNCaP cells the most resistant. Baicalin caused a 50% inhibition of DU145 cells at concentrations of 150 microM or above. The inhibition of proliferation of prostate cancer cells after a short period of exposure to baicalin was associated with induction by apoptosis, as evidenced by the typical nuclear fragmentation using Hoechst 33258 staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) labeling, DNA fragmentation, activation of caspase-3 and cleavage of poly-ADP-ribose polymerase (PARP). The results indicate that baicalin has direct anti-tumor effects on human prostate cancer cells.
黄酮类化合物黄芩苷（黄芩素7 - D - β - 葡萄糖醛酸苷），从黄芩（Huang Qin）的干燥根中分离得到，因其抗炎、解热和抗过敏作用而广泛应用于传统中药。在本研究中，我们研究了黄芩苷对几种人前列腺癌细胞系（包括DU145、PC - 3、LNCaP和CA - HPV - 10）的生长、活力及凋亡诱导的体外作用。用比色法3 -（4,5 - 二甲基噻唑 - 2 - 基）- 5 -（3 - 羧甲氧基苯基）- 2 -（4 - 磺基苯基）- 2H - 四唑（MTS）测定法对用黄芩苷处理2 - 4天后的细胞活力进行定量。结果表明，黄芩苷可抑制前列腺癌细胞的增殖。不同细胞系对黄芩苷的反应不同，DU145细胞最敏感，LNCaP细胞最耐药。在浓度为150 microM或更高时，黄芩苷对DU145细胞产生50%的抑制作用。短期暴露于黄芩苷后前列腺癌细胞增殖的抑制与凋亡诱导有关，这通过使用Hoechst 33258染色的典型核碎裂、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）标记、DNA片段化、caspase - 3的激活和聚ADP - 核糖聚合酶（PARP）的裂解得以证明。结果表明，黄芩苷对人前列腺癌细胞具有直接抗肿瘤作用。
