Hartikainen T, Ruuskanen J, Räty K, Von Wright A, Martikainen P J
Department of Environmental Sciences, University of Kuopio, Finland.
J Appl Microbiol. 2000 Oct;89(4):580-6. doi: 10.1046/j.1365-2672.2000.01150.x.
A bacterium (strain TJ330) capable of using carbon disulphide (CS2) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS2 and hydrogen sulphide (H2S) from air. Its physiology and taxonomy are described here. The strain oxidized CS2, H2S and elemental sulphur to sulphate chemolithotrophically. The rate of sulphate production was highest at pH 2. The maximum growth rate constant (micromax) using CS2 as a substrate was 3.9 x 10(-2) h(-1) (generation time 18 h) and the Monod constant (Ks) was 0.97-2.6 micromol l(-1) CS2 (74-198 microg l(-1)), corresponding to an equilibrium with 15-40 ppm CS2 in the headspace. The optimum growth temperature using elemental sulphur as a substrate was 28 degrees C. The strain bears morphological and physiological similarities to Thiobacillus thiooxidans, but the latter is incapable of oxidizing CS2. The strain TJ330 (DSM 8985) showed only 44.2 + 11.8% DNA homology with the type strain T. thiooxidans ATCC 19377, while its homology with T. ferrooxidans ATCC 23270 was 17.1 + 3.4%. The strain TJ 330 represents a high-affinity bacterium which can effectively remove low CS2 concentrations in an acid environment. These properties can be utilized in biotechnological purification applications.
从用于从空气中去除二硫化碳（CS₂）和硫化氢（H₂S）的泥炭生物滤池中分离出一种细菌（TJ330菌株），它能够在酸性环境中以二硫化碳作为唯一能源。本文描述了其生理特性和分类情况。该菌株通过化学无机营养将CS₂、H₂S和元素硫氧化为硫酸盐。在pH为2时硫酸盐生成速率最高。以CS₂为底物时的最大生长速率常数（μmₐₓ）为3.9×10⁻² h⁻¹（代时18小时），莫诺德常数（Ks）为0.97 - 2.6 μmol l⁻¹ CS₂（74 - 198 μg l⁻¹），对应于顶空中15 - 40 ppm CS₂的平衡浓度。以元素硫为底物时的最适生长温度为28℃。该菌株在形态和生理上与氧化硫硫杆菌相似，但后者不能氧化CS₂。TJ330菌株（DSM 8985）与氧化硫硫杆菌模式菌株ATCC 19377的DNA同源性仅为44.2 + 11.8%，而与氧化亚铁硫杆菌ATCC 23270的同源性为17.1 + 3.4%。TJ 330菌株是一种高亲和力细菌，能够在酸性环境中有效去除低浓度的CS₂。这些特性可用于生物技术净化应用。
