Guay R, Silver M
Can J Microbiol. 1975 Mar;21(3):281-8. doi: 10.1139/m75-040.
After a brief exposition to glucose, Thiobacillus acidophilus was isolated from a culture of iron-grown T. ferrooxidans. Physicochemical analysis of its DNA showed a G+C content of 62.9-63.2%. The new isolate grows best at 25-30 degrees C and at pH 3.0. Growth is possible between pH 1.5 and 6.0. Thiobacillus acidophilus is apparently strictly aerobic. Ammonium salts are the only suitable source of nitrogen. The bacterium is a facultative autotroph. In addition to elemental sulfur, it obtains energy from organic compounds such as D-glucose, D-galactose, D-fructose, D-mannitol, D-xylose, D-ribose, D-arabinose, L-arabinose, sucrose, sodium citrate, malic acid,dl-aspartic acid, and dl-glutamic acid. Thiobacillus acidophilus possesses the key enzymes of the tricarboxylic acid (TCA) cycle including NAD-and NADP-linked isocitric dehydrogenase and alpha-ketoglutarate dehydrogenase, and the key enzymes of the hexose monophosphate pathway (glucose-6-phosphate and 6-phosphogluconate dehydrogenase, and fructose 1,6-diphosphate aldolase). NADH oxidase has been found in particulate fraction of extracts. Rhodanese and thiosulfate oxidase have also been detected.
在短暂接触葡萄糖后，嗜酸硫杆菌是从铁生长的氧化亚铁硫杆菌培养物中分离出来的。对其DNA的物理化学分析表明，鸟嘌呤和胞嘧啶（G+C）含量为62.9 - 63.2%。新分离株在25 - 30摄氏度和pH值3.0时生长最佳。在pH值1.5至6.0之间也能生长。嗜酸硫杆菌显然是严格需氧的。铵盐是唯一合适的氮源。该细菌是兼性自养生物。除了元素硫外，它还能从有机化合物如D - 葡萄糖、D - 半乳糖、D - 果糖、D - 甘露醇、D - 木糖、D - 核糖、D - 阿拉伯糖、L - 阿拉伯糖、蔗糖、柠檬酸钠、苹果酸、dl - 天冬氨酸和dl - 谷氨酸中获取能量。嗜酸硫杆菌拥有三羧酸（TCA）循环的关键酶，包括NAD - 和NADP - 连接的异柠檬酸脱氢酶和α - 酮戊二酸脱氢酶，以及磷酸己糖途径的关键酶（葡萄糖 - 6 - 磷酸和6 - 磷酸葡萄糖酸脱氢酶，以及果糖1,6 - 二磷酸醛缩酶）。在提取物的颗粒部分发现了NADH氧化酶。也检测到了硫氰酸酶和硫代硫酸盐氧化酶。
