Jilek A, Engel E, Beier D, Lepperdinger G
Institute of Molecular Biology, Department of Biochemistry, Austrian Academy of Sciences, Billrothstr. 11, A-5020, Salzburg, Austria.
Gene. 2000 Oct 3;256(1-2):189-95. doi: 10.1016/s0378-1119(00)00355-3.
The genomic structure of the murine Bv8 gene was determined in 129/SvJ mouse, and the chromosomal localization was identified. Bv8 has first been characterized from skin secretion of the yellow-bellied toad, Bombina variegata. When injected into rat brain, this polypetide causes hyperalgesia. The murine Bv8 gene was shown to consist of four exons and was localized on chromosome 6 between the microsatellite markers D6Mit66 and D6Mit36 near the gene mem1, whereas the human counterpart was assigned to the non-syntenic region 3p21.1. Furthermore, the primary Bv8 transcript appeared to be alternatively spliced. The first variant contained all four exons yielding a product with a stretch highly enriched in basic amino acids in its central part. This domain is absent in the peptides from frog as well as in a splice variant expressed in mouse testis. A third variant gives rise to a truncated polypeptide.
在129/SvJ小鼠中确定了鼠源Bv8基因的基因组结构，并鉴定了其染色体定位。Bv8最初是从黄腹蟾蜍（Bombina variegata）的皮肤分泌物中鉴定出来的。当注射到大鼠脑中时，这种多肽会引起痛觉过敏。结果表明，鼠源Bv8基因由四个外显子组成，位于6号染色体上，在微卫星标记D6Mit66和D6Mit36之间，靠近基因mem1，而人类对应的基因位于非同源区域3p21.1。此外，Bv8的初级转录本似乎存在可变剪接。第一个变体包含所有四个外显子，产生的产物在其中心部分有一段高度富含碱性氨基酸的区域。青蛙的肽以及在小鼠睾丸中表达的剪接变体中都没有这个结构域。第三个变体产生一个截短的多肽。
