Sant V J, Sainani M N, Sami-Subbu R, Ranjekar P K, Gupta V S
National Chemical Laboratory, Plant Molecular Biology Unit, Division of Biochemical Sciences, 411 008, Pune, India.
Gene. 2000 Oct 17;257(1):157-66. doi: 10.1016/s0378-1119(00)00405-4.
Ty1-copia retrotransposon-like elements were amplified from Cicer species using primers derived from the conserved region of the reverse transcriptase gene. Two fragments, of size approximately 280bp and approximately 650 bp, were obtained, which on sequencing showed homology for the Ty1-copia reverse transcriptase region. Interestingly, the approximately 650 bp fragment showed two reverse transcriptase regions, one from Ty1-copia and the other from Tto1 element fused together. The copy number was high in the cultivated Cicer arietinum genome compared with the wild Cicer reticulatum. Genetic diversity among the Cicer species was investigated using the conserved primers which grouped the wild species and the cultivated C. arietinum separately.
使用源自逆转录酶基因保守区域的引物，从鹰嘴豆属物种中扩增出类Ty1 - copia逆转座子元件。获得了两个片段，大小分别约为280bp和约650bp，测序结果显示它们与Ty1 - copia逆转录酶区域具有同源性。有趣的是，约650bp的片段显示出两个逆转录酶区域，一个来自Ty1 - copia，另一个来自Tto1元件，二者融合在一起。与野生的网脉鹰嘴豆相比，栽培种鹰嘴豆基因组中的拷贝数较高。使用保守引物对鹰嘴豆属物种间的遗传多样性进行了研究，这些引物将野生种和栽培种鹰嘴豆分别归为不同类别。
