Rajput M K, Upadhyaya K C
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Mol Biol (Mosk). 2010 Sep-Oct;44(5):786-91.
Almost all active plant retroelements are known to be induced by various biotic and abiotic stresses. Here, we show the presence of transcriptionally active Ty1-copia like retroelements in chickpea and their induction in response to abiotic stress. Eight Ty1-copia retrotransposon like mRNA sequences were reverse transcribed, amplified, cloned and characterized from stressed plants. These mRNA sequences were not detected in chickpea plants grown under normal conditions. Basing on the similarity analysis, these RT transcript sequences were classified into three families. It is proposed that all sequences except CARE7 might be transcript sequences of functional retrotransposons. The mRNA sequence CARE3 shows 99% nucleotide identity to a genomic Ty1-copia like sequence present in the Genbank with accession no. AJ535883.
几乎所有活跃的植物反转录元件都已知是由各种生物和非生物胁迫诱导产生的。在此，我们展示了鹰嘴豆中存在转录活跃的类Ty1 - copia反转录元件以及它们对非生物胁迫的响应诱导情况。从受胁迫的植物中反转录、扩增、克隆并鉴定了8个类Ty1 - copia反转录转座子mRNA序列。在正常条件下生长的鹰嘴豆植株中未检测到这些mRNA序列。基于相似性分析，这些RT转录本序列被分为三个家族。有人提出，除CARE7外的所有序列可能是功能性反转录转座子的转录序列。mRNA序列CARE3与Genbank中登录号为AJ535883的一个类基因组Ty1 - copia序列具有99%的核苷酸同一性。
