Averbeck D, Chandra P, Biswas R K
Radiat Environ Biophys. 1975 Oct 2;12(3):241-52. doi: 10.1007/BF01327351.
Using monofunctional (Angelicin) and bifunctional furocoumarins (Psoralen and 8 Methoxypsoralen) plus 365 nm light it is shown that both damages, the induced monoadducts and/or crosslinks in DNA, provoke lethal and mutgenic effects in haploid and diploid cells of Saccharomyces cerevisiae. Bifunctional furocoumarins are about 20 times more effective in cell killing than Angelicin. Diploid cells are always more resistant than haploid cells. Dark repair (agar haolding) increases survival. This effect can be at least in part correlated to the release of bound material from DNA in dark repair conditions. Bifunctional psoralens (10 mug/ml) are at least 10-fold more effective in inducing nuclear gene black mutations (his- to HIS+) than Angelicin (10 mug/ml) plus 365 nm light or 254 nm ultraviolet light. In contrast cytoplasmic "petite" (delta-) mutations are about as frequently induced by Angelicin plus 365 nm light as by 254 nm UV light. Bifunctional furocoumarins are less effective. The frequency of cytoplasmic "petite" mutations per survivors decreases during dark repair conditions more efficiently after Angelicin than after Psoralen plus 365 nm light treatment.
使用单功能（当归素）和双功能呋喃香豆素（补骨脂素和8-甲氧基补骨脂素）加365纳米光，结果表明，DNA中诱导的单加合物和/或交联这两种损伤，都会在酿酒酵母的单倍体和二倍体细胞中引发致死和诱变效应。双功能呋喃香豆素在细胞杀伤方面的效果比当归素高约20倍。二倍体细胞总是比单倍体细胞更具抗性。暗修复（琼脂保存）可提高存活率。这种效应至少部分与暗修复条件下DNA结合物质的释放有关。双功能补骨脂素（10微克/毫升）在诱导核基因黑色突变（从his-到HIS+）方面比当归素（10微克/毫升）加365纳米光或254纳米紫外线至少有效10倍。相比之下，当归素加365纳米光诱导细胞质“小菌落”（δ-）突变的频率与254纳米紫外线诱导的频率大致相同。双功能呋喃香豆素的效果较差。在暗修复条件下，当归素处理后，每个存活细胞的细胞质“小菌落”突变频率比补骨脂素加365纳米光处理后更有效地降低。
