酿酒酵母体内染色体间关联的功能剖析。
Functional dissection of in vivo interchromosome association in Saccharomyces cerevisiae.
作者信息
Aragón-Alcaide L, Strunnikov A V
机构信息
Unit of Chromosome Structure and Function, NICHD, National Institutes of Health, 18T Library Drive, Bethesda, Maryland 20892-5430, USA.
出版信息
Nat Cell Biol. 2000 Nov;2(11):812-8. doi: 10.1038/35041055.
Abstract
Homologue pairing mediates both recombination and segregation of chromosomes at meiosis I. The recognition of nucleic-acid-sequence homology within the somatic nucleus has an impact on DNA repair and epigenetic control of gene expression. Here we investigate interchromosomal interactions using a non-invasive technique that allows tagging and visualization of DNA sequences in vegetative and meiotic live yeast cells. In non-meiotic cells, chromosomes are ordered in the nucleus, but preferential pairing between homologues is not observed. Association of tagged chromosomal domains occurs irrespective of their genomic location, with some preference for similar chromosomal positions. Here we describe a new phenomenon that promotes associations between sequence-identical ectopic tags with a tandem-repeat structure. These associations, termed interchromosome trans-associations, may underlie epigenetic phenomena.
摘要
同源配对介导减数分裂I期染色体的重组和分离。体细胞细胞核内核酸序列同源性的识别会影响DNA修复和基因表达的表观遗传控制。在这里，我们使用一种非侵入性技术研究染色体间的相互作用，该技术允许对营养生长和减数分裂的活酵母细胞中的DNA序列进行标记和可视化。在非减数分裂细胞中，染色体在细胞核中有序排列，但未观察到同源物之间的优先配对。标记的染色体结构域之间的关联与其基因组位置无关，对相似的染色体位置有一定偏好。在这里，我们描述了一种新现象，该现象促进具有串联重复结构的序列相同的异位标签之间的关联。这些关联，称为染色体间反式关联，可能是表观遗传现象的基础。
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