Takeuchi R, Suwa Y, Yamagishi T, Yonezawa Y
National Institute for Resources and Environment, AIST, MITI, Ecological Chemistry and Microbiology, Tsukuba, Ibaraki, Japan.
Chemosphere. 2000 Nov;41(9):1457-62. doi: 10.1016/s0045-6535(99)00521-4.
Transformation of all 19 chlorophenol (CP) isomers was investigated in a laboratory anaerobic methanogenic sludge that had not been exposed to synthetic chemicals. Concentration of CP was analyzed over time to calculate disappearance rate constants using first-order reaction kinetics and all possible CP degradation pathways were estimated. The rate constants ranged between 0.46 x 10(-3) and 0.161 day(-1). CPs were transformed via dechlorination. The chlorine atom at the ortho-position was the most easily dechlorinated, whereas dechlorination rate at the para-position was lowest. The overall pathways of CP transformation were much less diverse than that we previously found for contaminated sediment. The Dolfing hypothesis of microbial selection of the most thermodynamically favorable pathways was not applicable for CP transformation in this study as well as previous study performed by our group.
在未接触过合成化学品的实验室厌氧产甲烷污泥中，研究了19种氯酚（CP）异构体的转化情况。随着时间的推移分析CP的浓度，使用一级反应动力学计算消失速率常数，并估算所有可能的CP降解途径。速率常数范围在0.46×10⁻³至0.161天⁻¹之间。CPs通过脱氯作用进行转化。邻位的氯原子最容易脱氯，而对位的脱氯速率最低。CP转化的总体途径比我们之前在受污染沉积物中发现的要少得多。微生物选择热力学上最有利途径的多尔芬假设在本研究以及我们小组之前进行的研究中都不适用于CP的转化。
