Benestad H B, Boyum A, Warhuus K
Scand J Haematol. 1975 Oct;15(3):219-77. doi: 10.1111/j.1600-0609.1975.tb01077.x.
Bone marrow progenitor cells from anaemic W/WV mice were compared with normal +/+ cells utilizing the spleen colony, the agar colony and the diffusion chamber techniques. Spleen colony formation from W/WV cells was markedly defective, and more so for erythroid than for granuloid colonies. The progenitor cell concentration was apparently normal as measured by the two other techniques. The concentration of circulating progenitor cells also seemed to be normal. On the other hand, the cell formation per progenitor cell was subnormal in all three assay systems. The initial proliferative response of W/WV spleen colony-formers and agar colony-formers to short-term diffusion chamber culturing was apparently normal. The incorporation of 3H-thymidine, related to the number of proliferative granulocytes present in the chambers, also seemed to be normal. The results indicate that the W/WV defect is not limited to the multipotent stem cells. A possible interpretation is that it is the capacity for continued self-renewal of immature cells that is defective.
利用脾集落、琼脂集落和扩散盒技术，将贫血W/WV小鼠的骨髓祖细胞与正常+/+细胞进行比较。W/WV细胞形成脾集落的能力明显缺陷，红系集落比粒系集落更明显。用另外两种技术测量，祖细胞浓度显然正常。循环祖细胞浓度似乎也正常。另一方面，在所有三种检测系统中，每个祖细胞的细胞形成低于正常水平。W/WV脾集落形成细胞和琼脂集落形成细胞对短期扩散盒培养的初始增殖反应显然正常。与培养盒中增殖粒细胞数量相关的3H-胸腺嘧啶掺入似乎也正常。结果表明，W/WV缺陷不限于多能干细胞。一种可能的解释是，不成熟细胞持续自我更新的能力存在缺陷。
