脉冲场凝胶电泳在奥地利一个青年中心空肠弯曲菌暴发中的流行病学应用
Epidemiologic application of pulsed-field gel electrophoresis to an outbreak of Campylobacter jejuni in an Austrian youth centre.
作者信息
Lehner A, Schneck C, Feierl G, Pless P, Deutz A, Brandl E, Wagner M
机构信息
Institute of Milk Hygiene, Milk Technology and Food Science, University of Veterinary Medicine, Vienna, Austria.
出版信息
Epidemiol Infect. 2000 Aug;125(1):13-6. doi: 10.1017/s0950268899004318.
We report the first documented Campylobacter jejuni outbreak in an Austrian youth centre. Sixty-four children were involved of which 38 showed classical signs of campylobacter gastroenteritis. Since unpasteurized milk distributed by a local dairy was suspected to be the source of infection, stool samples were collected from 20 cows providing the milk. Five of the cows tested positive for C. jejuni. These isolates together with 37 clinical samples were compared by pulsed-field-gel electrophoresis (PFGE). The PFGE patterns, using the restriction endonucleases SmaI and SalI, were identical for the human and bovine isolates. This finding confirmed that the outbreak was caused by the consumption of unpasteurized milk contaminated with C. jejuni.
我们报告了奥地利一家青年中心首次有记录的空肠弯曲菌爆发事件。64名儿童受影响，其中38名出现空肠弯曲菌肠胃炎的典型症状。由于怀疑当地一家乳制品厂分发的未杀菌牛奶是感染源，于是从提供牛奶的20头奶牛身上采集了粪便样本。其中5头奶牛的空肠弯曲菌检测呈阳性。使用脉冲场凝胶电泳（PFGE）对这些分离株以及37份临床样本进行了比较。使用限制性内切酶SmaI和SalI得到的PFGE图谱显示，人类和牛的分离株完全相同。这一发现证实，此次疫情是由饮用受空肠弯曲菌污染的未杀菌牛奶所致。
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