亚甲蓝、靛胭脂溶液及自体红细胞悬液注射入大鼠后对粘连形成的影响

Effects of methylene blue, indigo carmine solution and autologous erythrocyte suspension on formation of adhesions after injection into rats.

作者信息

Gül A, Kotan C, Dilek I, Gül T, Taş A, Berktaş M

机构信息

Departments of Obstetrics and Gynecology, Faculty of Medicine, Yüzüncü Yil University, 65200, Van, Turkey.

出版信息

J Reprod Fertil. 2000 Nov;120(2):225-9. doi: 10.1530/jrf.0.1200225.

DOI:10.1530/jrf.0.1200225

PMID:11058437

Abstract

The aim of this study was to determine whether autologous erythrocyte suspension can be used as a dye for evaluation of tubal patency and whether it has any advantages over methylene blue or indigo carmine solutions. Reproductively healthy female nulliparous Wistar Albino rats (n = 30), aged 6 months, mass 165-195 g, were assigned randomly to three groups. Rats received a 1 ml i.p. injection of 5% (w/v) methylene blue solution (methylene blue group: n = 10), 5% (w/v) indigo carmine solution (indigo carmine group: n = 10) or 5% (v/v) fresh autologous erythrocyte suspension (autologous erythrocyte group: n = 10). At 4 weeks after injection, a small sterile opening was made in the peritoneal cavity of each rat. The cavity was rinsed once with TCM-199 to collect macrophages. The rinsed peritoneal contents were cultured overnight to evaluate macrophage activation. The peritoneal opening was expanded for evaluation of adhesion formation. Only one rat from the autologous erythrocyte group had intra-peritoneal adhesions (score 2), whereas all rats in the methylene blue group (score 1: n = 1; score 2: n = 4; score 3: n = 4; and score 4: n = 1) and seven rats in the indigo carmine group (score 1: n = 1; score 2: n = 2; score 3: n = 3; and score 4: n = 1) had intra-abdominal adhesions. Macrophage activity was observed in the cultured peritoneal contents collected from the methylene blue and indigo carmine groups but not from the autologous erythrocyte group. Adhesion formation could be due to macrophage activation caused by methylene blue and indigo carmine solutions. These results indicate that tubal patency can be observed by laparoscopy using autologous erythrocyte suspension. The results of this study are believed to be the first to indicate that a patient's own erythrocyte suspension could be used during observation of tubal patency by laparoscopy. However, further studies are required.

摘要

本研究的目的是确定自体红细胞悬液是否可作为一种染料用于评估输卵管通畅性，以及它是否比亚甲蓝或靛胭脂溶液具有任何优势。生殖健康的未生育雌性Wistar白化大鼠（n = 30），6月龄，体重165 - 195 g，被随机分为三组。大鼠腹腔注射1 ml 5%（w/v）亚甲蓝溶液（亚甲蓝组：n = 10）、5%（w/v）靛胭脂溶液（靛胭脂组：n = 10）或5%（v/v）新鲜自体红细胞悬液（自体红细胞组：n = 10）。注射后4周，在每只大鼠的腹腔做一个小的无菌开口。用TCM - 199冲洗腹腔一次以收集巨噬细胞。冲洗后的腹腔内容物培养过夜以评估巨噬细胞活化情况。扩大腹腔开口以评估粘连形成情况。自体红细胞组只有1只大鼠有腹腔粘连（评分2），而亚甲蓝组所有大鼠（评分1：n = 1；评分2：n = 4；评分3：n = 4；评分4：n = 1）以及靛胭脂组7只大鼠（评分1：n = 1；评分2：n = 2；评分3：n = 3；评分4：n = 1）有腹腔内粘连。在从亚甲蓝组和靛胭脂组收集的培养腹腔内容物中观察到巨噬细胞活性，而自体红细胞组未观察到。粘连形成可能归因于亚甲蓝和靛胭脂溶液引起的巨噬细胞活化。这些结果表明，使用自体红细胞悬液通过腹腔镜可观察输卵管通畅性。据信本研究结果首次表明，在通过腹腔镜观察输卵管通畅性期间可使用患者自身的红细胞悬液。然而，还需要进一步研究。