兔和鸡网织红细胞裂解物中碳酸酐酶的合成。

Synthesis of carbonic anhydrase in rabbit and chicken reticulocyte lysates.

作者信息

Cramer F, Gould H, Barlow S, Carter N

出版信息

Eur J Biochem. 1979 Mar 15;95(1):99-105. doi: 10.1111/j.1432-1033.1979.tb12943.x.

DOI:10.1111/j.1432-1033.1979.tb12943.x

Abstract

The synthesis of carbonic anhydrase, the second most abundant soluble protein of red blood cells, is shown to occur in rabbit and chicken reticulocyte lysates. This translation product was identified by chloroform/ethanol extraction, polyacrylamide gel electrophoresis in sodium dodecylsulphate and peptide mapping. In rabbit retic-locyte lysates, predominantly one of the two red cell isozymes, carbonic anhydrase I, is synthesised. The proportion of carbonic anhydrase synthesis (0.2-0.8% of total protein synthesis) in vitro is comparable to that (0.2-1.0%) in vivo for both rabbit and chicken reticulocytes.

摘要

红细胞中第二丰富的可溶性蛋白质碳酸酐酶的合成，已证实在兔和鸡网织红细胞裂解物中发生。该翻译产物通过氯仿/乙醇提取、十二烷基硫酸钠聚丙烯酰胺凝胶电泳和肽图谱分析进行鉴定。在兔网织红细胞裂解物中，主要合成两种红细胞同工酶之一的碳酸酐酶I。兔和鸡网织红细胞体外碳酸酐酶的合成比例（占总蛋白质合成的0.2 - 0.8%）与体内（0.2 - 1.0%）相当。