Beuzard Y, Rodvien R, London I M
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1022-6. doi: 10.1073/pnas.70.4.1022.
The initiation of globin synthesis in intact reticulocytes and in reticulocyte lysates is maintained by the addition of hemin. The specificity of this effect has been studied to determine whether it is restricted to hemoglobin and erythroid cells. In intact reticulocytes, hemin (500 muM) enhances the synthesis of carbonic anhydrase as well as of hemoglobin. Similar enhancement of protein synthesis is observed on addition of hemin (500 muM) to intact Krebs II ascites tumor cells, in cell-free extracts of these cells, added hemin (50 muM) increases endogenous protein synthesis and the translation of exogenous rabbit globin messenger RNA. These results provide evidence that the effect of hemin is not restricted to globin, and they suggest that hemin may enhance protein synthesis in at least some nonerythroid cells.
完整的网织红细胞和网织红细胞裂解物中珠蛋白合成的起始通过添加血红素得以维持。已对这种效应的特异性进行了研究，以确定它是否仅限于血红蛋白和红细胞。在完整的网织红细胞中，血红素（500μM）可增强碳酸酐酶以及血红蛋白的合成。向完整的克雷布斯II腹水肿瘤细胞中添加血红素（500μM）时，观察到蛋白质合成有类似增强，在这些细胞的无细胞提取物中，添加血红素（50μM）可增加内源性蛋白质合成以及外源兔珠蛋白信使核糖核酸的翻译。这些结果提供了证据，表明血红素的效应并不局限于珠蛋白，并且表明血红素可能至少在一些非红细胞中增强蛋白质合成。
