Grundy P L, Patel N, Harbuz M S, Lightman S L, Sharples P M
Department of Neurosurgery, Academic Centre, Frenchay Hospital, Bristol, UK.
Neuroreport. 2000 Oct 20;11(15):3381-4. doi: 10.1097/00001756-200010200-00023.
Brain-derived neurotrophic factor (BDNF) expression in rat hippocampus is increased after experimental traumatic brain injury (TBI) and may be neuroprotective. Glucocorticoids are important regulators of brain neurotrophin levels and are often prescribed following TBI. The effect of adrenalectomy (ADX) on the expression of BDNF mRNA in the hippocampus after TBI has not been investigated to date. We used fluid percussion injury (FPI) and in situ hybridization to evaluate the expression of BDNF mRNA in the hippocampus 4 h after TBI in adrenal-intact or adrenalectomized rats (with or without corticosterone replacement). FPI and ADX independently increased expression of BDNF mRNA. In animals undergoing FPI, prior ADX caused further elevation of BDNF mRNA and this upregulation was prevented by corticosterone replacement in ADX rats. These findings suggest that glucocorticoids are involved in the modulation of the BDNF mRNA response to TBI.
实验性创伤性脑损伤（TBI）后，大鼠海马中脑源性神经营养因子（BDNF）的表达增加，且可能具有神经保护作用。糖皮质激素是脑神经营养因子水平的重要调节因子，TBI后常被使用。迄今为止，尚未研究肾上腺切除术（ADX）对TBI后海马中BDNF mRNA表达的影响。我们采用液压冲击伤（FPI）和原位杂交技术，评估肾上腺完整或肾上腺切除大鼠（有无皮质酮替代）TBI后4小时海马中BDNF mRNA的表达。FPI和ADX分别增加BDNF mRNA的表达。在接受FPI的动物中，预先进行ADX会导致BDNF mRNA进一步升高，而ADX大鼠中的这种上调可通过皮质酮替代来预防。这些发现表明，糖皮质激素参与了对TBI的BDNF mRNA反应的调节。
