Nimmo G R, Schooneveldt J, O'Kane G, McCall B, Vickery A
Microbiology Department, Queensland Health Pathology Service, Princess Alexandra Hospital, Woolloongabba 4102, Australia.
J Clin Microbiol. 2000 Nov;38(11):3926-31. doi: 10.1128/JCM.38.11.3926-3931.2000.
Community-acquired methicillin-resistant Staphylococcus aureus (MRSA) susceptible to gentamicin has been reported in a number of countries in the 1990s. To study the acquisition of gentamicin-sensitive MRSA (GS-MRSA) in southeast Queensland and the relatedness of GS-MRSA to other strains of MRSA, 35 cases of infection due to GS-MRSA from October 1997 through September 1998 were examined retrospectively to determine the mode of acquisition and risk factors for MRSA acquisition. Thirty-one isolates from the cases were examined using a variety of methods (antibiotyping, phage typing, pulsed-field gel electrophoresis [PFGE] fingerprinting, and coagulase typing by restriction analysis of PCR products) and were compared with strains of local hospital-acquired gentamicin-resistant MRSA (GR-MRSA) and of Western Australian MRSA (WA-MRSA). Only 6 of 23 cases of community-acquired GS-MRSA had risk factors for MRSA acquisition. Twenty of 21 isolates from cases of community-acquired infection were found to be related by PFGE and coagulase typing and had similar phage typing patterns. Hospital- and nursing home-acquired GS-MRSA strains were genetically and phenotypically diverse. Community-acquired GS-MRSA strains were not related to nosocomial GR-MRSA or WA-MRSA, but phage typing results suggest that they are related to GS-MRSA previously reported in New Zealand.
20世纪90年代，一些国家报告了对庆大霉素敏感的社区获得性耐甲氧西林金黄色葡萄球菌（MRSA）。为了研究昆士兰东南部地区庆大霉素敏感MRSA（GS-MRSA）的获得情况以及GS-MRSA与其他MRSA菌株的相关性，对1997年10月至1998年9月期间35例由GS-MRSA引起的感染病例进行了回顾性研究，以确定MRSA的获得方式和危险因素。采用多种方法（抗生素分型、噬菌体分型、脉冲场凝胶电泳[PFGE]指纹图谱以及通过PCR产物限制性分析进行凝固酶分型）对其中31株菌株进行了检测，并与当地医院获得性耐庆大霉素MRSA（GR-MRSA）菌株和西澳大利亚MRSA（WA-MRSA）菌株进行了比较。23例社区获得性GS-MRSA病例中，只有6例具有MRSA获得的危险因素。从社区获得性感染病例中分离出的21株菌株中有20株通过PFGE和凝固酶分型显示具有相关性，并且噬菌体分型模式相似。医院和养老院获得的GS-MRSA菌株在基因和表型上具有多样性。社区获得性GS-MRSA菌株与医院获得性GR-MRSA或WA-MRSA无关，但噬菌体分型结果表明它们与先前在新西兰报道的GS-MRSA有关。
