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Sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci.对编码锰依赖性超氧化物歧化酶的基因进行测序以快速鉴定肠球菌的种类。
J Clin Microbiol. 2000 Jan;38(1):415-8. doi: 10.1128/JCM.38.1.415-418.2000.
2
Identification of clinically relevant viridans streptococci by analysis of transfer DNA intergenic spacer length polymorphism.通过分析转移DNA基因间间隔区长度多态性鉴定临床相关的草绿色链球菌
Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1591-8. doi: 10.1099/00207713-49-4-1591.
3
Proficiency of clinical laboratories in Spain in detecting vancomycin-resistant Enterococcus spp. The Spanish VRE Study Group.西班牙临床实验室检测耐万古霉素肠球菌属的能力。西班牙耐万古霉素肠球菌研究小组。
J Clin Microbiol. 1999 Jul;37(7):2148-52. doi: 10.1128/JCM.37.7.2148-2152.1999.
4
Determination of 16S rRNA sequences of enterococci and application to species identification of nonmotile Enterococcus gallinarum isolates.肠球菌16S rRNA序列的测定及其在鸡肠球菌非运动型分离株菌种鉴定中的应用。
J Clin Microbiol. 1998 Nov;36(11):3399-407. doi: 10.1128/JCM.36.11.3399-3407.1998.
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Multiple-drug resistant enterococci: the nature of the problem and an agenda for the future.多重耐药肠球菌：问题的本质与未来议程
Emerg Infect Dis. 1998 Apr-Jun;4(2):239-49. doi: 10.3201/eid0402.980211.
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Division of the genus Enterococcus into species groups using PCR-based molecular typing methods.使用基于聚合酶链反应（PCR）的分子分型方法将肠球菌属划分为不同的种组。
Microbiology (Reading). 1998 May;144 ( Pt 5):1171-1179. doi: 10.1099/00221287-144-5-1171.
7
Identification of clinically important species of Enterococcus within 1 day with randomly amplified polymorphic DNA (RAPD).利用随机扩增多态性DNA（RAPD）在1天内鉴定肠球菌的临床重要菌种。
Curr Microbiol. 1998 Jun;36(6):332-6. doi: 10.1007/s002849900318.
8
Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates.基于聚合酶链反应的DNA指纹技术在李斯特菌属鉴定中的比较及其在非典型李斯特菌分离株中的应用
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9
Clinical and epidemiological significance of enterococci intrinsically resistant to vancomycin (possessing the vanC genotype).对万古霉素天然耐药（具有vanC基因型）的肠球菌的临床和流行病学意义。
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Recent approaches on the taxonomy of the enterococci and some related microorganisms.肠球菌及一些相关微生物分类学的最新研究方法。
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应用tRNA基因间隔区PCR鉴定肠球菌属菌种。

Application of tRNA intergenic spacer PCR for identification of Enterococcus species.

作者信息

Baele M, Baele P, Vaneechoutte M, Storms V, Butaye P, Devriese L A, Verschraegen G, Gillis M, Haesebrouck F

机构信息

Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke, Belgium.

出版信息

J Clin Microbiol. 2000 Nov;38(11):4201-7. doi: 10.1128/JCM.38.11.4201-4207.2000.

DOI:10.1128/JCM.38.11.4201-4207.2000
PMID:11060090
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87563/
Abstract

tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans, while Enterococcus hirae was easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosus generated highly similar though distinctive patterns.

摘要

评估了tRNA基因间间隔区PCR（tDNA-PCR）在区分人和动物源肠球菌种类方面的实用性。对属于17种肠球菌的124株菌株进行了该技术操作，并产生了DNA片段，这些片段通过毛细管电泳进行分离。tDNA-PCR使我们能够区分所有测试的菌种。屎肠球菌与耐久肠球菌表现出微小但可重复的差异，而平肠球菌很容易区分。鸟肠球菌、恶臭肠球菌和棉子糖肠球菌产生了高度相似但又有区别的图谱。