应用tRNA基因间隔区PCR鉴定肠球菌属菌种。
Application of tRNA intergenic spacer PCR for identification of Enterococcus species.
作者信息
Baele M, Baele P, Vaneechoutte M, Storms V, Butaye P, Devriese L A, Verschraegen G, Gillis M, Haesebrouck F
机构信息
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke, Belgium.
出版信息
J Clin Microbiol. 2000 Nov;38(11):4201-7. doi: 10.1128/JCM.38.11.4201-4207.2000.
Abstract
tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans, while Enterococcus hirae was easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosus generated highly similar though distinctive patterns.
摘要
评估了tRNA基因间间隔区PCR（tDNA-PCR）在区分人和动物源肠球菌种类方面的实用性。对属于17种肠球菌的124株菌株进行了该技术操作，并产生了DNA片段，这些片段通过毛细管电泳进行分离。tDNA-PCR使我们能够区分所有测试的菌种。屎肠球菌与耐久肠球菌表现出微小但可重复的差异，而平肠球菌很容易区分。鸟肠球菌、恶臭肠球菌和棉子糖肠球菌产生了高度相似但又有区别的图谱。
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J Clin Microbiol. 2000 Jan;38(1):415-8. doi: 10.1128/JCM.38.1.415-418.2000.
2
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Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1591-8. doi: 10.1099/00207713-49-4-1591.
3
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J Clin Microbiol. 1999 Jul;37(7):2148-52. doi: 10.1128/JCM.37.7.2148-2152.1999.
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6
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7
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Int J Syst Bacteriol. 1998 Jan;48 Pt 1:127-39. doi: 10.1099/00207713-48-1-127.
9
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