Quednau M, Ahrné S, Petersson A C, Molin G
Laboratory of Food Hygiene, Department of Food Technology, Lund University, Sweden.
Curr Microbiol. 1998 Jun;36(6):332-6. doi: 10.1007/s002849900318.
The use of randomly amplified polymorphic DNA (RAPD) for rapid, reliable, and easily interpreted identification of enterococci was evaluated. Nineteen type strains of Enterococcus, 12 reference strains, and 114 clinical isolates of Enterococcus were analyzed. Discrimination was obtained between most type strains, the exceptions being Ent. casseliflavus and Ent. flavescens, which had relatively similar RAPD-profiles. Ent. faecalis and Ent. faecium were readily separated, and Ent. gallinarum and Ent. durans could also be identified. Extracts to be used in the polymerase chain reaction (PCR) were prepared directly from agar plate colonies, which made it possible to complete the identification procedure in one day. RAPD was proved to be a fast and reliable method for identification of most Enterococcus spp. of clinical significance.
对随机扩增多态性DNA（RAPD）用于快速、可靠且易于解读的肠球菌鉴定进行了评估。分析了19株肠球菌模式菌株、12株参考菌株以及114株肠球菌临床分离株。大多数模式菌株之间能够区分开，但例外的是格氏肠球菌和微黄肠球菌，它们具有相对相似的RAPD图谱。粪肠球菌和屎肠球菌很容易区分开，鹑鸡肠球菌和耐久肠球菌也能够被鉴定出来。用于聚合酶链反应（PCR）的提取物直接从琼脂平板菌落制备，这使得在一天内完成鉴定程序成为可能。RAPD被证明是鉴定大多数具有临床意义的肠球菌属的快速且可靠的方法。
